Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts

Published: April 30, 2023

Abstract

Source: Noel P. et al. Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts. J. Vis. Exp. (2017)

This video explains the 3D magnetic bioprinting of co-cultured pancreatic cancer cells and fibroblasts. Pancreatic cancer cells and activated pancreatic fibroblast cells are co-cultured and magnetized using a biocompatible nanoparticle assembly, to generate spheroids, under the influence of magnetic forces.

Protocol

1.Culture of Pancreatic Cancer 3D Spheroids using Magnetic Bioprinting Using standard aseptic tissue culture technique, culture cells of interest in a T75 flask to a confluency of 70-80% in appropriate growth media. NOTE: Typically, 5-7 × 106 cells from a 70-80% confluent T75 flask were harvested. Two different cell types were used in this study – Patu8902 (pancreatic tumor cells) and PS1 cells (activated pancreatic stellate cells). Both cell lines were cultured in…

Açıklamalar

The authors have nothing to disclose.

Materials

0.05% Trypsin EDTA  Sigma  25300-054
96 well cell repellant plate  USA Scientific/ Griener Bio-One  655970 spheroid growth plate
Cellometry Cell counting slides  Nexcelom  CHT4-SD100-002
n3D Magnetic Spheroid drive  Nano3D Bioscience  655841 Part of Spheroid Bioprinting Kit
n3D Magnetic Spheroid holding
drive
Nano3D Bioscience  655841 Part of Spheroid Bioprinting Kit
n3D NanoShuttle-PL  Nano3D Bioscience  655841 Part of Spheroid Bioprinting Kit:
store at 4 °C
Patu8902 cells  Laboratory Stock pancreatic ductal adenocarcinoma
cells
PS1 cells Laboratory Stock activated pancreatic stellate cells
RPMI1640  Gibco  11875-093  growth media for cells, spheroids

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Bu Makaleden Alıntı Yapın
Magnetically Bioprinted 3D Spheroid Co-cultures: A Method for Co-culturing Cancerous Cells and Fibroblasts. J. Vis. Exp. (Pending Publication), e20315, doi: (2023).

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