Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients
Published: April 30, 2023
Abstract
Source: Giallombardo, M., et al. Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool. J. Vis. Exp. (2016).
The plasma-isolated exosomes can be used as a non-invasive and repeatable way for the detection and follow-up of the biomarkers for early detection and disease progression. This video describes the protocol for the separation of exosomes from the plasma samples of non-small cell lung cancer patients, which could be used for DNA and protein extractions.
Protocol
1. Exosome Isolation Note: The isolation of exosomes from plasma samples, was performed with a commercial kit, according to manufacturer's protocol and by adding RNAse treatment: (all these steps must be performed with personal and environment protective equipments and following specific legal procedure for manipulation of biological samples). Take 1 ml of plasma of each sample, stored at -80 °C, and place it on ice. Centrifuge the sample at 2,00…
Açıklamalar
The authors have nothing to disclose.
Materials
| Total exosome isolation kit (from plasma) | Invitrogen | 4484450 | Use Proteinase K treatment |
| Ribonuclease A | Sigma-Aldrich | R4875-100MG | |
| RNAspin Illustra mini kit 50 | GE HealthCare | 25-0500-71 |
Bu Makaleden Alıntı Yapın
Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients. J. Vis. Exp. (Pending Publication), e20250, doi: (2023).