In Vivo Antitumor Efficacy Analysis of PDT: A Technique to Determine the Phototoxic Potential of a Photosensitizer in Tumor Bearing Mice
Published: April 30, 2023
Abstract
Source: Chang, J. E., et al. Anticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles. J. Vis. Exp. (2016).
Photodynamic therapy (PDT) is a non-invasive and non-surgical method for lung cancer treatment. Photosensitizers selectively accumulate in tumor tissue and lead to tumor cell death in the presence of oxygen and the proper wavelength of light. This video describes the in vivo anti-tumor efficacy analysis using photosensitizer containing nanoparticles in a tumor-bearing mouse, to determine the phototoxic potential of the photosensitizer.
Protocol
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Synthesis of Hyaluronic Acid-Ceramide (HACE) Solubilize 12.21 mmol of hyaluronic acid (HA) oligomer and 9.77 mmol of tetra-n-butylammonium hydroxide (TBA) in 60 ml of double-distilled water (DDW). Stir for 30 min. To synthesize the DS-Y30 linker, dissolve 8.59 mmol of DS-Y30 ceramide and 9.45 mmol of triethylamine in 25 ml …
Açıklamalar
The authors have nothing to disclose.
Materials
| oligo hyaluronic acid | Bioland Co., Ltd. | ||
| DS-Y30 (ceramide 3B; mainly Noleoyl-phytosphingosine) | Doosan Biotech Co., Ltd. | ||
| adipic acid dihydrazide | Sigma Aldrich | A0638 | |
| N-(3-dimethylaminopropyl)-N′- ethylcarbodiimide | Sigma Aldrich | 39391 | |
| 4-(chloromethyl)benzoyl chloride | Sigma Aldrich | 270784 | |
| Paclitaxel | Taihua Corporations | ||
| syringe filter | Sartorius Stedim Biotech GmbH | 17762 | 15 mm, RC, PP, 0.45 µm |
| RPMI-1640 | Gibco Life Technologies, Inc. | 11875 | |
| Penicillin–streptomycin | Gibco Life Technologies, Inc. | 15070 | |
| Fetal bovine serum | Gibco Life Technologies, Inc. | 16140071 | |
| Celite (Filter agent) | Sigma Aldrich | 6858 | See step 1.4 |
Bu Makaleden Alıntı Yapın
In Vivo Antitumor Efficacy Analysis of PDT: A Technique to Determine the Phototoxic Potential of a Photosensitizer in Tumor Bearing Mice. J. Vis. Exp. (Pending Publication), e20249, doi: (2023).