Drosophila Egg Collection and Dechorionation: A Method to Remove the Outermost Egg Layer
Published: April 30, 2023
Abstract
Source: Koyle, M. L., et al. Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions. J. Vis. Exp. (2016).
The Drosophila embryo is surrounded by protective outer membranes. To gain access to the embryo, the chorion–the outermost of the membranes–is often removed. This video describes a commonly used method of dechorionation using sodium hypochlorite. The example protocol demonstrates the procedure while employing sterile techniques necessary for axenic or gnotobiotic animal rearing.
Protocol
This protocol is an excerpt from Koyle et al., Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions, J. Vis. Exp. (2016). 1. Prepare Egg-laying Cages Make grape-juice agar plates by microwaving 100 ml water, 10 g brewer's yeast, 10 g glucose, and 1 g of agar. Bring to a boil 3 times in step 1.1 and add 10 g of frozen grape juice concentrate to increase visibility of eggs on the agar …
Materials
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Drosophila Egg Collection and Dechorionation: A Method to Remove the Outermost Egg Layer. J. Vis. Exp. (Pending Publication), e20119, doi: (2023).