JOVE JOURNAL

İmmünoloji ve Enfeksiyon

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Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Rachel E. Ham*¹, Austin R. Smothers*^1,2, Kylie L. King¹, Justin M. Napolitano¹, Theodore J. Swann³, Lesslie G. Pekarek⁴, Mark A. Blenner*^1,5,6, Delphine Dean*^1,2

¹Center for Innovative Medical Devices and Sensors (REDDI Lab),Clemson Üniversitesi, ²Biyomühendislik Bölümü,Clemson Üniversitesi, ³Swann Medicine, ⁴Student Health Services,Clemson Üniversitesi, ⁵Department of Chemical & Biomolecular Engineering,Clemson Üniversitesi, ⁶Department of Chemical & Biomolecular Engineering,University of Delaware

Journal (Immunology and Infection)

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle (VLP)-Based Vaccines Using a Capture Assay

Jamila Franca Rosengarten*^1,2, Stefanie Schatz*^1,2, Jörn Stitz¹

¹Research Group Pharmaceutical Biotechnology,TH Köln - University of Applied Sciences, ²Institute of Technical Chemistry,Leibniz University Hannover

Journal (Immunology and Infection)

Chemical Cartography Approaches to Study Trypanosomatid Infection

Danya A. Dean*^1,2, Jacob J. Haffner*^2,3, Mitchelle Katemauswa¹, Laura-Isobel McCall^1,2,4

¹Department of Chemistry and Biochemistry,University of Oklahoma, Norman, ²Laboratories of Molecular Anthropology and Microbiome Research,University of Oklahoma, Norman, ³Department of Anthropology,University of Oklahoma, Norman, ⁴Department of Microbiology and Plant Biology,University of Oklahoma, Norman

Journal (Immunology and Infection)

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

Chantelle E Bowers¹, Virginia L Calder¹, John Greenwood¹, Malihe Eskandarpour¹

¹UCL Institute of Ophthalmology,University College London

Journal (Immunology and Infection)

Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis

Sarah John¹, Oliver H. Bell², Leslie Wilson¹, David A. Copland², Kathryn L. Pepple¹

¹Department of Ophthalmology,Washington Üniversitesi, ²Academic Unit of Ophthalmology, Translational Health Sciences,University of Bristol

Journal (Immunology and Infection)

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Xiaoyan Chang*¹, Keru Zhou*¹, Yanguang Liu^1,2, Lian Duan³, Lichun Zhang², Guoliang Zhang³, Haikun Wang⁴, Guiqin Wang¹

¹Nanjing Advanced Academy of Life and Health, ²Institute of Animal Bio-technology,Jilin Academy of Agricultural Sciences, ³National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital,Southern University of Science and Technology, ⁴CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences,University of Chinese Academy of Sciences

Journal (Immunology and Infection)

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Anthos Christofides^1,2, Carol Cao^1,2,3, Rinku Pal^1,2, Halil I. Aksoylar^1,2, Vassiliki A. Boussiotis^1,2

¹Division of Hematology-Oncology,Beth Israel Deaconess Medical Center, Harvard Medical School, ²Tıp Fakültesi,Beth Israel Deaconess Medical Center, Harvard Medical School, ³Harvard College

Journal (Immunology and Infection)

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

Lichen Zhang¹, Rong Huang¹, Liaoxun Lu¹, Rui Fu¹, Guo Guo¹, Yanrong Gu¹, Zhuangzhuang Liu¹, Le He¹, Marie Malissen², Yinming Liang¹

¹The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine,Xinxiang Medical University, ²Centre d’Immunologie de Marseille-Luminy,Aix Marseille Université

Journal (Immunology and Infection)

Isolation of Primary Mouse Lung Endothelial Cells

Erika Wong^1,2, Nina Nguyen², Judith Hellman²

¹Department of Pediatrics, Division of Critical Care,UCSF Benioff Children's Hospital, ²Department of Anesthesia and Perioperative Care,University of California, San Francisco

Journal (Immunology and Infection)

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Shammy Raj¹, Lei Lu², Larry D. Unsworth¹

¹Department of Chemical and Materials Engineering, Donadeo Innovation Centre for Engineering,Alberta Üniversitesi, ²School of Life Science and Engineering,Southwest Jiaotong University

Journal (Immunology and Infection)

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Desarey Morales Vasquez¹, Kevin Chiem¹, Jesus Silvas¹, Jun-Gyu Park¹, Chengjin Ye¹, Luis Martínez-Sobrido¹

¹Texas Biomedical Research Institute

Journal (Immunology and Infection)

Plaquing of Herpes Simplex Viruses

Lauren A. Sadowski*¹, Gregory M. Lesko*¹, Chad Suissa*¹, Rista Upadhyay*^1,2, Prashant J. Desai³, Barry J. Margulies^1,4

¹Towson University Herpes Virus Lab, Department of Biological Sciences,Towson University, ²Towson University Department of Chemistry,Towson University, ³Department of Oncology,Johns Hopkins University School of Medicine, ⁴Molecular Biology, Biochemistry, and Bioinformatics Program,Towson University

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