JOVE JOURNAL

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Journal (Developmental Biology)

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Afif Hedhly¹, Hannes Vogler¹, Christof Eichenberger¹, Ueli Grossniklaus¹

¹Department of Plant and Microbial Biology, Zurich-Basel Plant Science Center,University of Zurich

Journal (Developmental Biology)

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Li Xiao¹, Hisashi Okamura², Yasuo Kumazawa²

¹Department of Pharmacology,The Nippon Dental University, ²Department of Oral and Maxillofacial Surgery,The Nippon Dental University Hospital

Journal (Developmental Biology)

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

Tomoko Yamazaki^1,2, Wenling Li¹, Yoh-Suke Mukouyama¹

¹Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, ²Earle A. Chiles Research Institute, Robert W. Franz Cancer Center,Providence Portland Medical Center

Journal (Developmental Biology)

Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks

George Merz¹, Valerie Schwenk¹, Ruchit Shah², Carolyn Salafia^1,2, Phillip Necaise², Michael Joyce¹, Tom Villani³, Michael Johnson³, Nick Crider³

¹The Institute for Basic Research,The New York State Office for People with Developmental Disabilities, ²Placental Analytics LLC, ³Visikol Inc.

Journal (Developmental Biology)

Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea

Divya A. Shiroor¹, Tisha E. Bohr¹, Carolyn E. Adler¹

¹Department of Molecular Medicine, College of Veterinary Medicine,Cornell University

Journal (Developmental Biology)

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

Irene U. Wacker^1,2, Lisa Veith³, Waldemar Spomer^2,4, Andreas Hofmann^2,4, Marlene Thaler⁵, Stefan Hillmer⁶, Ulrich Gengenbach^2,4, Rasmus R. Schröder^1,2,3

¹Cryo Electron Microscopy, Centre for Advanced Materials,Universität Heidelberg, ²Heidelberg Karlsruhe Research Partnership (HEiKA), ³Cryo Electron Microscopy, BioQuant,Universitätsklinikum Heidelberg, ⁴Institute for Automation and Applied Computer Science,Karlsruhe Institute of Technology (KIT), ⁵Carl Zeiss Microscopy GmbH, ⁶Electron Microscopy Core Facility,Universität Heidelberg

Journal (Developmental Biology)

Single-cell Photoconversion in Living Intact Zebrafish

Lauren Green^1,2, Cody J. Smith^1,2

¹Department of Biological Sciences,Notre Dame Üniversitesi, ²Center for Stem Cells and Regenerative Medicine,Notre Dame Üniversitesi

Journal (Developmental Biology)

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Qinggang Dai*¹, Yujiao Han*², Furong Xie*¹, Xuhui Ma³, Zhan Xu², Xiao Liu¹, Weiguo Zou², Jun Wang¹

¹Department of Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research Center of Stomatology, ²State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology,University of Chinese Academy of Sciences, ³Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital,Shanghai Jiaotong University School of Medicine

Journal (Developmental Biology)

Live Cell Imaging of Chromosome Segregation During Mitosis

Prajakta Varadkar¹, Kazuyo Takeda², Brenton McCright¹

¹Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research,US Food and Drug Administration, ²Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research,US Food and Drug Administration

Journal (Developmental Biology)

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

David G. Valcarce^1,2, Vanesa Robles^2,3

¹Department of Molecular Biology,University of León, ²INDEGSAL,University of León, ³Planta de Cultivos el Bocal,Spanish Institute of Oceanography (IEO)

Journal (Developmental Biology)

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Mahlet D. Mersha¹, Karla R. Sanchez², Murali K. Temburni², Harbinder S. Dhillon²

¹Department of Neurology,Johns Hopkins School of Medicine, ²Department of Biological Sciences,Delaware State University

Journal (Developmental Biology)

A Neural Network-Based Identification of Developmentally Competent or Incompetent Mouse Fully-Grown Oocytes

Federica Cavalera¹, Mario Zanoni¹, Valeria Merico¹, Thi Thu Hien Bui^1,2, Martina Belli^1,3, Lorenzo Fassina⁴, Silvia Garagna¹, Maurizio Zuccotti¹

¹Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani",University of Pavia, ²Institute of Biotechnology,University of Helsinki, ³Department of Reproductive Medicine,University of California San Diego, ⁴Dipartimento di Ingegneria Industriale e dell'Informazione,University of Pavia

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