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Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Daniel L. Kober*^1,2,3, Zeynep Yurtsever*^2,3,4,5, Thomas J. Brett^2,3,5,6,7

¹Molecular Microbiology and Microbial Pathogenesis Program,Washington Üniversitesi Tıp Fakültesi, ²İç Hastalıkları Anabilim Dalı,Washington Üniversitesi Tıp Fakültesi, ³Drug Discovery Program in Pulmonary and Critical Care Medicine,Washington Üniversitesi Tıp Fakültesi, ⁴Biochemistry Program,Washington Üniversitesi Tıp Fakültesi, ⁵Center for the Investigation of Membrane Excitability Diseases,Washington Üniversitesi Tıp Fakültesi, ⁶Department of Biochemistry and Molecular Biophysics,Washington Üniversitesi Tıp Fakültesi, ⁷Department of Cell Biology and Physiology,Washington Üniversitesi Tıp Fakültesi

Journal (Biology)

Purification and Refolding to Amyloid Fibrils of (His)₆-tagged Recombinant Shadoo Protein Expressed as Inclusion Bodies in E. coli

Qiaojing Li¹, Charles-Adrien Richard¹, Mohammed Moudjou¹, Jasmina Vidic¹

¹Virologie et Immunologie Moléculaires,INRA

Journal (Biology)

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

Anna K. Schellhaus*¹, Adriana Magalska*², Allana Schooley¹, Wolfram Antonin¹

¹Friedrich Miescher Laboratory,Max Planck Society, ²Nencki Institute of Experimental Biology,Polish Academy of Sciences

Journal (Biology)

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

Heather A. Paul¹, Megan C. Hallam², Raylene A. Reimer^1,2

¹Department of Biochemistry and Molecular Biology, Cumming School of Medicine,University of Calgary, ²Faculty of Kinesiology,University of Calgary

Journal (Biology)

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

Lala Aliyeva-Schnorr¹, Lu Ma^1,2, Andreas Houben¹

¹Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), ²School of Biological and Chemical Sciences,Queen Mary University of London

Journal (Biology)

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Priyanka Dutta*¹, Christina Lehmann*¹, Devang Odedra*¹, Deepika Singh*¹, Christian Pohl¹

¹Buchmann Institute for Molecular Life Sciences and Institute of Biochemistry II, School of Medicine,Goethe University

Journal (Biology)

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Shona A. Mookerjee^1,2, Martin D. Brand^1,2

¹Touro University California College of Pharmacy, ²Buck Institute for Research on Aging

Journal (Biology)

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

Takumi Higaki¹

¹Graduate School of Frontier Sciences,The University of Tokyo

Journal (Biology)

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

David Razafsky¹, Chloe Potter¹, Didier Hodzic¹

¹Department of Ophthalmology and Visual Sciences,Washington University in St. Louis School of Medicine

Journal (Biology)

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Edith Honvo-Houéto¹, Sandrine Truchet¹

¹Jouy-en-Josas Centre,INRA

Journal (Biology)

Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule

Jeffrey N. Schellinger¹, Aylin R. Rodan¹

¹İç Hastalıkları Anabilim Dalı,Teksas Üniversitesi Southwestern Tıp Merkezi

Journal (Biology)

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Yuan-Jyue Chen¹, Sundipta D. Rao¹, Georg Seelig^1,2

¹Department of Electrical Engineering,Washington Üniversitesi, ²Department of Computer Science & Engineering,Washington Üniversitesi

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