JOVE JOURNAL

Biyokimya

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Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion

Fatima Cabral¹, Colton M. Miller¹, Katrina M. Kudrna¹, Blake E. Hass¹, Jocelyn G. Daubendiek¹, Brianna M. Kellar¹, Edward N. Harris¹

¹Department of Biochemistry,University of Nebraska

Journal (Biochemistry)

Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems

Charlotte A. Scarff¹, Martin J. G. Fuller², Rebecca F. Thompson², Matthew G. Iadanza¹

¹Astbury Centre for Structural Molecular Biology,University of Leeds, ²Astbury Biostructure Laboratory,University of Leeds

Journal (Biochemistry)

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

April Maschmann¹, Cody Masters¹, Melissa Davison¹, Joshua Lallman¹, Drew Thompson¹, Kristy L. Kounovsky-Shafer¹

¹Kimya Bölümü,University of Nebraska - Kearney

Journal (Biochemistry)

Mouse Adipose Tissue Collection and Processing for RNA Analysis

Paul Tan^1,2, Émilie Pepin^1,4, Julie L. Lavoie^1,3,4

¹Centre de Recherche du Centre Hospitalier,Université de Montréal, ²Biyokimya ve Moleküler Tıp Bölümü,Université de Montréal, ³Department of Kinesiology,Université de Montréal, ⁴Montreal Diabetes Research Center

Journal (Biochemistry)

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Markus Götz¹, Philipp Wortmann¹, Sonja Schmid^1,2, Thorsten Hugel¹

¹Fiziksel Kimya Enstitüsü,University of Freiburg, ²Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology

Journal (Biochemistry)

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

Aaron J. Paredes¹, Hilda M. Alfaro-Valdés¹, Christian A.M. Wilson¹

¹Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas,Universidad de Chile

Journal (Biochemistry)

Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

Fabrice Gorrec¹, Jan Löwe¹

¹Laboratory of Molecular Biology,Medical Research Council

Journal (Biochemistry)

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

Matthew B. O'Rourke^1,2, Matthew P. Padula², Caine Smith³, Priscilla Youssef⁴, Stuart Cordwell¹, Paul Witting⁴, Greg Sutherland³, Ben Crossett¹

¹Mass Spectrometry Core Facility,University of Sydney, ²Proteomics Core Facility,University of Technology Sydney, ³Neuropathology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney, ⁴Redox Biology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney

Journal (Biochemistry)

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Yu-Hua Lo¹, Monica C. Pillon¹, Robin E. Stanley¹

¹Signal Transduction Laboratory, National Institutes of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Journal (Biochemistry)

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Henrick Horita¹, Andy Law¹, Kim Middleton¹

¹R&D Department,Cytoskeleton Inc.

Journal (Biochemistry)

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Wenhui Lyu¹, Jennifer Selinski¹, Lu Li¹, David A. Day², Monika W. Murcha³, James Whelan¹, Yan Wang¹

¹ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science,La Trobe University, ²School of Biological Sciences,Flinders University, ³ARC Centre of Excellence in Plant Energy Biology,University of Western Australia

Journal (Biochemistry)

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Carine Beaupere¹, Rosalyn B. Chen¹, William Pelosi¹, Vyacheslav M. Labunskyy¹

¹Dermatoloji Anabilim Dalı,Boston University School of Medicine

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