JOVE JOURNAL

Biyokimya

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Journal (Biochemistry)

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Keri L Colabroy¹, Katlyn Mayer¹

¹Kimya Bölümü,Muhlenberg College

Journal (Biochemistry)

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

Markus Nachbar¹, Jorrit Maul¹, Matthias Stein¹, Hermann Wätzig¹

¹Institute of Medicinal and Pharmaceutical Chemistry,TU Braunschweig

Journal (Biochemistry)

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

Xiongwei Cai^1,2,3, Yi Zheng¹, Nancy A. Speck²

¹Division of Experimental Hematology,Cincinnati Children's Hospital Medical Center, ²Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute,Perelman School of Medicine, University of Pennsylvania, ³Department of Obstetrics and Gynecology, Southwest Hospital,Third Military Medical University

Journal (Biochemistry)

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)

Xiaoying Qi*¹, Yunyun Zhang*², Yuan Zhang¹, Tianhui Ni³, Wenfeng Zhang², Chunhua Yang¹, Jia Mi^1,4, Jiandi Zhang^2,3, Geng Tian¹

¹Medicine and Pharmacy Research Center,Binzhou Medical University, ²Yantai Zestern Biotechnique Co. LTD, ³Precision Medicine research center,Binzhou Medical University, ⁴Department of Chemistry - BMC,Uppsala University

Journal (Biochemistry)

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Sayan Chakraborty¹, Krittin Trihemasava¹, Guozhou Xu¹

¹Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences,North Carolina State University

Journal (Biochemistry)

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Tanja D. Becke^1,2,3, Stefan Ness², Stefanie Sudhop^1,3, Hermann E. Gaub³, Markus Hilleringmann², Arndt F. Schilling*⁴, Hauke Clausen-Schaumann*^1,3

¹Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, ²FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, ³Center for Nano Science,Ludwig-Maximilians-Universität München, ⁴Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen

Journal (Biochemistry)

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Valentina Iadevaia*¹, Ana M. Matia-González*¹, André P. Gerber¹

¹Dept. of Microbial Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences,University of Surrey

Journal (Biochemistry)

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Michael J. Stephenson¹, James Reed¹, Bastiaan Brouwer¹, Anne Osbourn¹

¹John Innes Centre

Journal (Biochemistry)

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Alicia Ventresca Miller¹, Ricardo Fernandes^1,2, Anneke Janzen¹, Ayushi Nayak¹, Jillian Swift¹, Jana Zech¹, Nicole Boivin¹, Patrick Roberts¹

¹Max Planck Institute for the Science of Human History, ²School of Archaeology,University of Oxford

Journal (Biochemistry)

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Daniela Baitan^1,2, Robin Schubert^2,3, Arne Meyer¹, Karsten Dierks¹, Markus Perbandt^2,3, Christian Betzel^2,3

¹Xtal Concepts GmbH, ²Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation c/o DESY,University of Hamburg, ³The Hamburg Center for Ultrafast Imaging,University of Hamburg

Journal (Biochemistry)

Detection and Isolation of Apoptotic Bodies to High Purity

Thanh Kha Phan¹, Ivan KH Poon¹, Georgia K Atkin-Smith¹

¹Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science,La Trobe University

Journal (Biochemistry)

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Alexandra J. Machen¹, Pierce T. O'Neil¹, Bradley L. Pentelute², Maria T. Villar¹, Antonio Artigues¹, Mark T. Fisher¹

¹Biyokimya ve Moleküler Biyoloji Anabilim Dalı,University of Kansas Medical Center, ²Kimya Bölümü,Massachusetts Institute of Technology

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