JOVE JOURNAL

Biyokimya

Showing 37 - 48 in 759 videos.

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Journal (Biochemistry)

Inverted Fluorescence Microscopy and Data Analysis to Assess Drug Effects on cAMP in HASM Cells

Isabella Cattani-Cavalieri, Jordyn Margolis, Camelia Anicolaesei, Francisco J. Nuñez, Rennolds S. Ostrom

¹Department of Biomedical and Pharmaceutical Sciences,Chapman University School of Pharmacy

Journal (Biochemistry)

Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ

Isabella Cattani-Cavalieri, Jordyn Margolis, Camelia Anicolaesei, Francisco J. Nuñez, Rennolds S. Ostrom

¹Department of Biomedical and Pharmaceutical Sciences,Chapman University School of Pharmacy

Journal (Biochemistry)

Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70

Hloniphani Rachel Ncube, Unarine Dali, Tendamudzimu Harmfree Dongola, Addmore Shonhai

¹Department of Biochemistry & Microbiology,University of Venda

Journal (Biochemistry)

Transformation of E. coli Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity

Hloniphani Rachel Ncube, Unarine Dali, Tendamudzimu Harmfree Dongola, Addmore Shonhai

¹Department of Biochemistry & Microbiology,University of Venda

Journal (Biochemistry)

Analysis of Hsp70 Chaperone Activity Using SDS PAGE and Western Blot Analyses of Transformed E. coli Protein

Hloniphani Rachel Ncube, Unarine Dali, Tendamudzimu Harmfree Dongola, Addmore Shonhai

¹Department of Biochemistry & Microbiology,University of Venda

Journal (Biochemistry)

In Situ X-ray Crystallographic Data Collection Using the Beamline at Diamond Light Source

James Sandy, Halina Mikolajek², Amy J. Thompson, Juan Sanchez-Weatherby, Michael A Hough²

¹Diamond Light Source Ltd,Harwell Science and Innovation Campus, ²Research Complex at Harwell,Harwell Science and Innovation Campus

Journal (Biochemistry)

Crystallization and In Situ Room Temperature Data Collection Using the Crystallization Facility at Harwell and Beamline VMXi, Diamond Light Source

James Sandy, Halina Mikolajek², Amy J. Thompson, Juan Sanchez-Weatherby, Michael A Hough²

¹Diamond Light Source Ltd,Harwell Science and Innovation Campus, ²Research Complex at Harwell,Harwell Science and Innovation Campus

Journal (Biochemistry)

Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

Russell Spencer-Smith^2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

Journal (Biochemistry)

Transfection of 293FT Cells with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo Expression Constructs

Russell Spencer-Smith^2,3

Journal (Biochemistry)

Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

Russell Spencer-Smith^2,3

Journal (Biochemistry)

Sequence Diversification of Protein-Peptide Interface with Pepspec

Héctor Cruz², Alejandro Llanes³, Patricia L. Fernández³

¹Facultad de Ciencias y Tecnología,Universidad Tecnológica de Panamá (UTP), ²Centro de Biología Molecular y Celular de Enfermedades,Instituto de Investigaciones Científicas y Servicios de Alta Tecnología AIP (INDICASAT AIP), ³Sistema Nacional de Investigación de Panamá (SNI)

Journal (Biochemistry)

Computational Alanine Scanning Mutagenesis of Protein-Peptide Interface

Héctor Cruz², Alejandro Llanes³, Patricia L. Fernández³

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