Construction of Out&#45;of&#45;Equilibrium Metabolic Networks in Nano&#45; and Micrometer&#45;Sized Vesicles

Jelmer Coenradij; Eleonora Bailoni; Bert Poolman

doi:10.3791/66627

Dergi

Biyokimya

Lipid Vesicle Encapsulation Protocol for Complex Protein Networks

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Jelmer Coenradij¹, Eleonora Bailoni¹, Bert Poolman¹

¹Department of Biochemistry,Groningen Üniversitesi

Özet

We present a protocol for reconstituting membrane proteins and encapsulating enzymes and other water-soluble components in lipid vesicles of sub-micrometer and micrometer size.