Dergi
In Situ Nucleosome Reconstitution for Single-Molecule Studies
September 06, 2024
, , ,

Özet

This article presents a detailed experimental procedure for reconstituting nucleosome-containing DNA tethers for single-molecule correlative force and fluorescence microscopy. It further describes several downstream experiments that can be conducted to visualize the binding behavior of chromatin-interacting proteins and analyze changes in the physical properties of nucleosomes.