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Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

October 13, 2023

活小鼠精子顶体钙动力学和胞吐作用的实时成像

从AcroSensE小鼠模型中收集和清洗精子以进行实时成像

建立单细胞兴奋剂递送系统，实时监测活小鼠精子

将显微镜与兴奋剂递送系统相结合，监测AcroSensE模型小鼠的活精子

Roy Cohen^1,2, Danielle M. Sosnicki¹, Melissa A. White¹, Jacquelyn L. Nelson¹, Chinatsu Mukai¹, Alexander J. Travis^1,2

¹Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Özet

这里描述的AcroSensE小鼠模型和活细胞成像方法为研究精子顶体亚细胞区室中的钙动力学以及它们如何调节导致膜融合和顶体胞吐作用的中间步骤提供了一种新方法。

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