Dergi
Biyokimya
Characterization of Ternary Complex Formation in PROTACs Using Biophysical Assays

Characterization of Ternary Complex Formation in PROTACs Using Biophysical Assays

January 12, 2024

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

01:09 min
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1,911 Views

Isothermal Titration Calorimetry for Evaluating PROTACS-Induced Ternary Complex Formation

01:16 min
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174 Views

Biolayer Interferometry (BLI) for Evaluating PROTACS-Induced Ternary Complex Formation

01:23 min
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179 Views

Surface Plasmon Resonance (SPR) for Evaluating PROTACS-Induced Ternary Complex Formation

03:36 min
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170 Views
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1Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Özet

Here we describe protocols for the biophysical characterization of ternary complex formation induced by proteolysis targeting chimeras (PROTACS) that involve the ubiquitin ligases Von Hippel-Lindau E3 ligase (VHL) and Cereblon (CRBN). Biophysical methods illustrated herein include surface plasmon resonance (SPR), biolayer interferometry (BLI), and isothermal titration calorimetry (ITC).

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