Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay

Tobia Fantoni; Michele Bissoli; Chiara Stefani; Mauro Voi; Alexandrina Dabija; Rebecca Casula; Domenico Luca Minafra; Julys da Fonseca Palmeira; Enrique Roberto Arga&#241;araz; Martin Mayora-Neto; Nigel J. Temperton; Donato Zipeto; Alessandra Ruggiero

doi:10.3791/65658

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Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses

November 21, 2023

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

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SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay

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Tobia Fantoni*¹, Michele Bissoli*¹, Chiara Stefani¹, Mauro Voi¹, Alexandrina Dabija¹, Rebecca Casula¹, Domenico Luca Minafra¹, Julys da Fonseca Palmeira², Enrique Roberto Argañaraz², Martin Mayora-Neto³, Nigel J. Temperton³, Donato Zipeto¹, Alessandra Ruggiero¹

¹Department of Neuroscience, Biomedicine and Movement,University of Verona, ²Laboratory of Molecular Neurovirology, Faculty of Health Science,University of Brasília, ³Viral Pseudotype Unit, Medway School of Pharmacy,Universities of Kent and Greenwich at Medway

Özet

Pseudotyped viruses (PVs) are replication-defective virions that are used to study host-virus interactions under safer conditions than handling authentic viruses. Presented here is a detailed protocol that shows how SARS-CoV-2 PVs can be used to test the neutralizing ability of patients’ serum after COVID-19 vaccination.

Assessing Serum Neutralization Against SARS-CoV-2 with Pseudotyped Viruses

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

SARS-CoV-2 Pseudotype Virus Production and Serum Neutralization Assay

Özet

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