Using Avian Skin Explants to Study Tissue Patterning and Organogenesis

Tingxin Jiang; Maeve Secor; Rusty Lansford; Randall B. Widelitz; Cheng Ming Chuong

doi:10.3791/65580

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Tingxin Jiang¹, Maeve Secor², Rusty Lansford³, Randall B. Widelitz¹, Cheng Ming Chuong¹

¹Patoloji Bölümü,Keck School of Medicine of University of Southern California, ²Molecular and Computational Biology,University of Southern California, ³Department of Radiology,Keck School of Medicine of University of Southern California

Özet

Here we describe protocols for three types of avian embryonic skin explant cultures that can be used to examine tissue interactions, 4D imaging timelapse movie (3D plus time), global or local perturbation of molecular function, and systems biology characterization.