Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Rafeeh Dinani; Emmy Manders; Michiel Helmes; Lili Wang; Bjorn C. Knollmann; Diederik W. D. Kuster; Jolanda van der Velden

doi:10.3791/65326

Dergi

Biyoloji

Real-Time Measurements of Calcium and Contractility Parameters in hiPSC-CMs

May 26, 2023

Author Spotlight: Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

01:21 min

•

1,433 Views

Contractility Measurements in hiPSC-CMs

02:30 min

•

267 Views

Calcium Transient Measurements in hiPSC-CMs

01:29 min

•

188 Views

Contractility and Calcium Transient Measurements Data Analysis

01:40 min

•

258 Views

Rafeeh Dinani^1,2, Emmy Manders^1,2,3, Michiel Helmes^1,2,3, Lili Wang⁴, Bjorn C. Knollmann⁴, Diederik W. D. Kuster^1,2, Jolanda van der Velden^1,2

¹Division of Physiology, Amsterdam UMC,Vrije University, ²Heart Failure & Arrhythmias,Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology,Vanderbilt School of Medicine

Özet

Here, an established method is described to perform contractility and calcium measurements in human induced pluripotent stem cell-derived cardiomyocytes using an optics-based platform. This platform enables researchers to study the effect of mutations and the response to various stimuli in a rapid and reproducible manner.