Ein Durchflussbelichtungssystem für die Bewertung Schwebstoffe Auswirkungen auf Leben im Wasser
Summary
A robust and flexible flow-through exposure system designed to maintain sediment in suspension is presented. The system is used to investigate the effects of suspended sediment on various aquatic species and life stages in the laboratory.
Abstract
This paper describes the Fish Larvae and Egg Exposure System (FLEES). The flow-through exposure system is used to investigate the effects of suspended sediment on various aquatic species and life stages in the laboratory by using pumps and automating delivery of sediment and water to simulate suspension of sediment. FLEES data are used to develop exposure-response curves between the effects on aquatic organisms and suspended sediment concentrations at the desired exposure duration. The effects data are used to evaluate management practices used to reduce the interactions between aquatic organisms and anthropogenic causes of suspended sediments. The FLEES is capable of generating total suspended solids (TSS) concentrations as low as 30 to as high as 800 mg/L, making this system an ideal choice for evaluating the effects of TSS resulting from many activities including simulating low ambient levels of TSS to evaluating sources of suspended sediments from dredging operations, vessel traffic, freshets, and storms.
Introduction
Ausbaggerarbeiten verwenden mechanische Methoden, um Bodensedimenten aus Häfen und Fahrrinnen entfernen. Während der Entnahme wird ein Teil des gestörten Sediments in die Wassersäule suspendiert, potentiell und dass das eine Quelle für körperliche Belastung für Wasserlebewesen. Darüber hinaus ausgesetzt wird, werden das Sediment kann, bevor sie sich aus der Wassersäule entfernt von der Dredge durch Umgebungsbedingungen transportiert. Die Kombination dieser beiden Mechanismen bedeutet, dass ein Wasserorganismen in der Nähe eines Betriebs Baggers auftritt können Schwebstoffe ausgesetzt und erleiden nachteilige Auswirkungen. Um die Behebung solcher Bedenken, Umwelt Fenster (saisonal Ausbaggern Einschränkungen) werden routinemäßig als Management – Praxis zu reduzieren oder zu beseitigen Risiko von potenziell schädlichen Auswirkungen von Schwebstoffen aus Baggerarbeiten auf Wasserressourcen 1,2.
Umwelt Fenster werden am häufigsten gefährdet zu schützen gegründet, bedroht oderkommerziell wertvolle Arten wie Zander (Sander Vitreus) und Amerikanische Auster (Crassostrea virginica) 3. Die tragende Begründung für die Verhängung von Umwelt Fenster konzentriert sich oft auf , wie Dredge Aktivitäten können potenziell stören körperlich (zB Schwebstoffe) eines Tieres Fähigkeit , einen bestimmten Teil seiner Lebensgeschichte zu vervollständigen. Die Lebensstadien häufig zitiert sind Eier und Larven für Routen Migration offen zu halten für anadromous Art 3. Allerdings gibt es nur begrenzte Informationen artspezifischen biologischen Wirkungen relevant Schwebstoffe 4,5 betreffend zur Verfügung zur Verwendung von Umwelt Fenster als Instrument des Risikomanagements informieren.
Aus diesen Gründen wurde die entflieht entworfen, gebaut und verwendet, um die Suspension von Sedimenten zu simulieren und ihre Auswirkungen auf die frühen Lebensstadien von Wasserorganismen zu bestimmen. Entflieht Studien verwenden feinkörnige Sedimentpartikel (dh überwiegend Schluffe,Tone und feinen Sand), die am wahrscheinlichsten in Suspension zu bleiben und am weitesten von der Quelle zu migrieren. Die entflieht der Lage ist, Testfischeier und Larven, aber es kann auch zur Aufnahme anderer Wasserorganismen nachgerüstet werden, ist es eine einzigartige Fähigkeit macht. Die resultierenden biologischen Antwortdaten können dann verwendet werden, um die Auswirkungen von Schwebstoffen zu beurteilen. Die folgenden Verfahren geben einen Überblick darüber, wie kann die Technologie wiederholbar Schwebstoffe Konzentrationen und Effekte Daten aufgebaut und betrieben werden unter Verwendung verschiedener Wasserarten zu erhalten.
Protocol
Representative Results
Discussion
Die entflieht – Technologie verbessert die auf bestehenden Methoden 4,9 durch die Aufrechterhaltung und Schwebstoffe Kontrolle über einen weiten Bereich von Belichtungszeiten und Schwebstoffe Konzentrationen eines automatisierten, computergesteuerten System. Die Technologie ist flexibel, so dass es verwendet werden kann, um die Auswirkungen von Schwebstoffen auf mehrere Wassertierarten und Lebensstadien von unterschiedlichen Größen von Eiern an Erwachsene je nach Art zu bewerten. In Zukunft ist die Technol…
Disclosures
The authors have nothing to disclose.
Acknowledgements
This research was funded by the U.S. Army Corps of Engineers Dredging Operations and Environmental Research Program, Todd Bridges, Director. Permission was granted by the Chief of Engineers to publish this material.
Materials
| Parts List for one FLEES Module, Water Bath, and Aquarium | |||
| post, wood – used to build module (cut to 78 in) | Local vendor | N/A | Quantity: 4 Size: 4 in x 4 in x 8 ft |
| plywood, marine grade – fastened to wooden posts about 18 in off ground – for holding water bath (60 in x 42 in) | Local vendor | N/A | Quantity: 1 Size: 3/4 in x 4 ft x 8 ft |
| plywood – fastened on top of wooden posts – for holding pipes, solenoids and electrical (60 in x 42 in) | Local vendor | N/A | Quantity: 1 Size: 1/4 in x 4 ft x 8 ft |
| stud, wood – used to brace plywood and wooden posts (cut to fit) | Local vendor | N/A | Quantity: 4 Size: 2 in x 4 in x 96 in |
| tank, fiberglass – water bath with two drains: 1) to supply chiller; and 2) to drain water | Hydro Composites, LLC, Stockdale, TX, USA | FBT-226 | Quantity: 1 Size: 150-gal |
| chiller, water with self contained pump – for water bath; chiller sits under module | Remcor Products Co., Glendale Heights, IL, USA | CFF-500 | Quantity: 1 Size: 1/2 hp |
| tank, domed bottom – FLEES aquaria – sit inside water bath | United States Plastic Corp, Lima, OH, USA | 5197 | Quantity: 5 Size: 19 L |
| tank, stand – acrylic stand, 12 in x 12 in x 6 in – to hold aquaria | custom built by ERDC shops | N/A | Quantity: 5 Size: custom |
| urethane, liquid- to seal carboy plug and prevent leaking | Forsch Polymer Corporation, Englewood, CO | 60A Liquid Urethane | Quantity: 5 Size: epoxy kit |
| pump, magnetic drive – to suspend sediment in each aquarium | March Manufacturing Inc., Glenview, IL, USA | MDX-3-1/2 115 v | Quantity: 5 Size: 28 liter per min |
| light, LED – installed over water bath | C2 Development, Inc., Ames, IA, USA | Hydra 26 | Quantity: 2 Size: based on area to light |
| pipe, PVC schedule 40 – installed in drain of water bath to control water level | Local vendor | N/A | Quantity: – Size: 1 in |
| fittings, bulkhead – for aquaria/water bath connections to pumps, drains, water and slurry lines | Lifegard Aquatics, Cerritos, CA, USA | R270900 | Quantity: 20 Size: 1/2 in FPT x FPT |
| fittings, quick-disconnect, male pipe threaded inserts – insert in tank bulkhead | Cole-Parmer, Vernon Hills, IL, USA | EW-31303-36 | Quantity: 10 Size: 1/2 in MPT |
| fittings, quick-disconnect, valved hose barbs – connection between aquarium and insert in tank bulkhead | Cole-Parmer, Vernon Hills, IL, USA | EW-31303-11 | Quantity: 10 Size: 1/2 in |
| fittings, black HDPE threaded elbow – for aquaria vinyl tube connections to slurry/water line and pump | United States Plastic Corp, Lima, OH, USA | 62043 | Quantity: 20 Size: 1/2 NPT x 1/2 in Hose ID |
| fittings, black HDPE threaded adapter – for connections between pump and tank bulkhead | United States Plastic Corp, Lima, OH, USA | 62017 | Quantity: 10 Size: 1/2 NPT x 1/2 in Hose ID |
| tube, vinyl – connect slurry/water line to aquaria and to connect pumps to aquaria | Local vendor | N/A | Quantity: 25 ft Size: 1/2 in ID |
| tube, vinyl – connect to aquaria drains inserts and water bath drain | Local vendor | N/A | Quantity: 25 ft Size: 5/8 in ID |
| clamp, hose, stainless steel – to clamp vinyl tube to hose barbs | Local vendor | N/A | Quantity: 40 Size: #8 |
| Name | Company | Catalog Number | Comments |
| Parts list for Slurry System | |||
| chiller, water with self contained pump – sits off to side of slurry tank | Remcor Products Co., Glendale Heights, IL, USA | CFF-500 | Quantity: 1 Size: 1/2 hp |
| 125 gallon open top cone bottom tank w/Stand – 42 in x 35 in – contains the water and sediment to make slurry | United States Plastic Corp, Lima, OH, USA | 8586 | Quantity: 1 Size: 125 gal |
| Cover for 125 gallon tank | United States Plastic Corp, Lima, OH, USA | 8935 | Quantity: 1 Size: 42 in x 35 in |
| valve, PVC – connect tank drain to pump – isolate for maintenance | local plumbing vendor | N/A | Quantity: 2 Size: 1-1/2 in |
| pump, double diaphragm mounted on stand – used to recirculate slurry | Wilden-pumps.co.uk & Air Pumping Ltd., Essex, UK | P2/PPPP/WF/WF/PTV/400 | Quantity: 1 contact distributor |
| sensor, optical backscatter – measure NTU in slurry tank | Campbell Scientific, Logan, UT, USA | OBS-3+ | Quantity: 1 Size: 0-1000 NTU |
| pipe, PVC Schedule 40 – to recirculate slurry | local plumbing vendor | N/A | Quantity: 20 ft Size: 1 in |
| pipe, flexible PVC – fitted with union and used to connect to next module | local plumbing vendor | N/A | Quantity: 10 ft Size: 1 in |
| union, PVC Schedle 80 Socket – connect slurry line with next module | local plumbing vendor | N/A | Quantity: 8 Size: 1/2 in |
| solenoid, plastomatic (normally closed) – introduce slurry | Plast-O-Matic Valves, Inc., Cedar Grove, NJ, USA | EASYMT4V12R24-PV | Quantity: 5 Size: 1/2 in NPT threaded, 24 VAC contact distributor |
| fitting, PVC tee – connect slurry pipe with solenoid | local plumbing vendor | N/A | Quantity: 5 Size: 1 in x 1 in x 1 in slip x slip x FIPT |
| fittings, 1 in PVC ball valve threaded – shut off for slurry delivery to solenoid/water lines | local plumbing vendor | N/A | Quantity: 7 Size: 1/2 in |
| fittings, 1 in PVC union threaded – connect slurry solenoid to shut off valve | local plumbing vendor | N/A | Quantity: 5 Size: 1/2 in |
| tube, vinyl – connection between water solenoid and slurry solenoid | Local vendor | N/A | Quantity: 50 ft Size: 1/4" ID |
| Name | Company | Catalog Number | Comments |
| Parts list for Water System | |||
| chiller, water with self contained pump – sits off to side of reservoir | Remcor Products Co., Glendale Heights, IL, USA | CFF-500 | Quantity: 1 Size: 1/2 hp |
| 125 gallon open top cone bottom tank w/Stand – 42 in x 35 in – contains the water and sediment to make slurry | United States Plastic Corp, Lima, OH, USA | 8586 | Quantity: 1 Size: 125 gal |
| Cover for 125 gallon tank | United States Plastic Corp, Lima, OH, USA | 8935 | Quantity: 1 Size: 42 in x 35 in |
| valve, PVC – connect tank drain to water pump | local plumbing vendor | N/A | Quantity: 2 Size: 1 in |
| pump, magnetic drive, in-line use – used to recirculate water to aquaria and chiller | Little Giant, Fort Wayne, IN, USA | 3-MD-SC | Quantity: 1 Size: 1/12 hp |
| solenoid, alco – introduce water | discontinued; ASCO, Florham Park, NJ,USA for similar | N/A | Quantity: 5 Size: 24 v, 1/4 in NIPT |
| fittings, black HDPE reducer connector – connect 1/4 in hose water line from solenoid to 1/2 in hose | local plumbing vendor | N/A | Quantity: 5 Size: 1/2 in hose ID x 1/4 in hose ID |
| fittings, black HDPE tee – connect 1/2 in hose water line and slurry to aquaria | local plumbing vendor | N/A | Quantity: 5 Size: 1/2 in NPT x 1/2 in hose ID x 1/2 in hose ID |
| fittings, street elbow | local plumbing vendor | N/A | Quantity: 5 Size: 1/2 in 90° MIPT x FIPT |
| fittings, PVC threaded pipe nipples – connect union fittings with solenoids and other connections | local plumbing vendor | N/A | Quantity: 12 Size: 1/2 in |
| fittings, union threaded – connect slurry/water lines with next module | local plumbing vendor | N/A | Quantity: 6 Size: 1 in PVC |
| fittings, reducer bushing – connect to reducer tee in water line | local plumbing vendor | N/A | Quantity: 5 Size: 1/2 in male by 1/4 in female FIPT |
| fittings, threaded pipe nipples – connection between bushing and water solenoid | local plumbing vendor | N/A | Quantity: 5 Size: 4 in long x 1/4 in |
| pipe, PVC – make connections between tank, pump and chiller | local plumbing vendor | N/A | Quantity: 5 ft Size: Schedule 40 |
| Name | Company | Catalog Number | Comments |
| Parts list for Sensors, Data Acquisition Device, and Computer Software | |||
| software, LabView | National Instruments, Austin, Texas, USA | LabView 2015 Base | Quantity: 1 Size: N/A |
| SCXI-1001 12-Slot Chassis, U.S. 120 VAC | National Instruments, Austin, Texas, USA | 776571-01 | Quantity: 1 Size: N/A |
| SCXI 1100 – 32-Channel, ±10 V Analog Input Module | National Instruments, Austin, Texas, USA | 776572-00 | Quantity: 1 Size: N/A |
| SCXI 1303 – Terminal block designed for high-accuracy thermocouple measurements | National Instruments, Austin, Texas, USA | 777687-03 | Quantity: 2 Size: N/A |
| SCXI 1102B – 32-Channel Thermocouple/Voltage Input Module | National Instruments, Austin, Texas, USA | 776572-02B | Quantity: 1 Size: N/A |
| SCXI 1161 – General-Purpose Relay Module | National Instruments, Austin, Texas, USA | 776572-61 | Quantity: 6 Size: N/A |
| SCXI 1300 – General-Purpose Voltage Module | National Instruments, Austin, Texas, USA | 777687-00 | Quantity: 1 Size: N/A |
| PCMCIA Card DAQCARD-AI-16E-4 | National Instruments, Austin, Texas, USA | N/A – legacy | Quantity: 1 Size: N/A used cards available online |
| sensor, optical backscatter – measure NTU in each aquarium | Campbell Scientific Inc., Logan, UT, USA | OBS-3+ | Quantity: 5 Size: 0-1000 NTU |
References
- National Research Council (NRC). A process for setting, managing, and monitoring environmental windows for dredging projects. Marine Board, Transportation Research Board, Special Report 262. , (2001).
- Suedel, B. C., Kim, J., Clarke, D. G., Linkov, I. A risk-informed decision framework for setting environmental windows for dredging projects. Sci Total Environ. 403, 1-11 (2008).
- Reine, K. J., Dickerson, D. D., Clarke, D. G. Environmental windows associated with dredging operations. DOER Technical Notes Collection. ERDC TN DOER-E2. , (1998).
- Wilber, D. H., Clarke, D. G. Biological effects of suspended sediments: A review of suspended sediment impacts on fish and shellfish with relation to dredging activities in estuaries. N Am J Fish Manag. 21, 855-875 (2001).
- Suedel, B. C., Lutz, C. H., Clarke, J. U., Clarke, D. G. The effects of suspended sediment on walleye (Sander vitreus) eggs. J. Soils Sediments. 12, 995-1003 (2012).
- Travis, J., Kring, J. . LabVIEW for Everyone: Graphical Programming Made Easy and Fun (National Instruments Virtual Instrumentation Series). , (2006).
- Hazardous Waste Test Methods/SW-846 On-line. Office of Solid Waste and Emergency Response (OSWER) Available from: https://www.epa.gov/hw-sw846/sw-846-compendium (2016)
- Plumb, R. H. . Procedure for handling and chemical analysis of sediment and water samples, EPA/CE-81-1. , (1981).
- Clarke, D. G., Wilber, D. H. . Assessment of potential impacts of dredging operations due to sediment resuspension. , (2000).
- Suedel, B. C., Clarke, J. U., Lutz, C. H., Clarke, D. G., Godard-Codding, C., Maul, J. Suspended sediment effects on walleye (Sander vitreus). J. Great Lakes Res. 40, 141-148 (2014).
- Wilkens, J. L., Katzenmeyer, A. W., Hahn, N. M., Hoover, J. J., Suedel, B. C. Laboratory test of suspended sediment effects on short-term survival and swimming performance of juvenile Atlantic Sturgeon (Acipenser oxyrinchus oxyrinchus). J. Appl. Ichthy. 31, 984-990 (2015).
- Suedel, B. C., Clarke, J. U., Wilkens, J., Lutz, C. H., Clarke, D. G. The effects of a simulated sediment plume on eastern oyster (Crassostrea virginica) survival, growth, and condition. Estuaries and Coasts. 38 (2), 578-589 (2015).
- Bilotta, G. S., Brazier, R. E. Understanding the influence of suspended solids on water chemistry and aquatic biota. Water Res. 42, 2849-2861 (2008).
- Reine, K., Clarke, D., Dickerson, C., Pickard, S. Assessment of potential impacts of bucket dredging plumes on walleye spawning habitat in Maumee Bay, Ohio. Proceedings of the 18th World Dredging Congress (WODCON XVIII). , (2007).
