動電位腐食試験
Summary
Here, we present a protocol to set up and run an in vitro potentiodynamic corrosion system to analyze pitting corrosion for small metallic medical devices.
Abstract
Different metallic materials have different polarization characteristics as dictated by the open circuit potential, breakdown potential, and passivation potential of the material. The detection of these electrochemical parameters identifies the corrosion factors of a material. A reliable and well-functioning corrosion system is required to achieve this.
Corrosion of the samples was achieved via a potentiodynamic polarization technique employing a three-electrode configuration, consisting of reference, counter, and working electrodes. Prior to commencement a baseline potential is obtained. Following the stabilization of the corrosion potential (Ecorr), the applied potential is ramped at a slow rate in the positive direction relative to the reference electrode. The working electrode was a stainless steel screw. The reference electrode was a standard Ag/AgCl. The counter electrode used was a platinum mesh. Having a reliable and well-functioning in vitro corrosion system to test biomaterials provides an in-expensive technique that allows for the systematic characterization of the material by determining the breakdown potential, to further understand the material’s response to corrosion. The goal of the protocol is to set up and run an in vitro potentiodynamic corrosion system to analyze pitting corrosion for small metallic medical devices.
Introduction
電気化学的技術は、材料の電気化学的特性を得るために、迅速かつ比較的安価な方法を提供します。これらの技術は、制御された電気化学的妨害1-5への電荷移動プロセスの応答を観察することにより、金属の腐食を検出する能力に主に基づいています。体内環境内の金属インプラントの腐食が原因で生体適合性材料の完全性6上の有害な影響に重要です。体内でのインプラントの腐食に寄与する主な要因は、金属イオン7-11の放出増加につながる表面酸化物の溶解です。これは、局所的に、しかし、インプラント10,12-28の早期故障につながる可能性の全身性の影響で見つけることができる有害な生物学的反応、になります。
試験片の腐食特性を生成偏光スキャンから予測されますポテンショスタットによります。偏光スキャンは、金属基板の運動と腐食パラメータの外挿を可能にします。スキャン中に、電気活性種の酸化又は還元は電荷移動および反応物または生成物の移動によって制限することができます。これらの要因はすべて、偏光スキャンによってカプセル化されています。したがって、複数のサイクル全体で信頼性と再現性偏光スキャンを生成するシステムを持つことの重要性は非常に重要です。この原稿の主な焦点は、十分に機能動電位腐食系を得るために取ら原理と手順を特定のプロトコルを提供することです。
Protocol
Representative Results
Discussion
ステンレス鋼試料から生成された偏光スキャンは、信頼性が高く、29再現性の両方でうまく機能して腐食システムを示す文献に見られるスキャンと相関きれい連続プロットを示しました。動電位孔食電位の再現性が悪いが、確率過程29によって特徴づけられる潜在的なビーイング孔食で、数百ミリボルトの普及と識別されます。これは一般的に、温度、ハロゲン化物含有量と?…
Disclosures
The authors have nothing to disclose.
Acknowledgements
The authors had no funding provided for this study.
Materials
| Potentiostat | Metrohm | PGSTAT101 | |
| Ag/AgCl reference electrode, shielded | Metrohm | 6.0729.100 | |
| Electrode shaft | Metrohm | 6.1241.060 | |
| Polisher Forcipol 1v | Metkon | 3602 | |
| Clindrical flask 700mL | SciLabware | FR700F | |
| Reaction lid | SciLabware | MAF2/41 | |
| Dichloromethane | Sigma-Aldrich | MKBR7629V | use under a fumehood. Wear protective clothing |
| Thermo / HAAKE D Series Immersion Circulators | Haake |
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