在鼠害丙烯酸树脂模塑基头固定技术
Summary
Traditional head fixation techniques have used metallic frames attached on the skull as an interface for head fixation apparatus. This protocol demonstrates a frameless, acrylic resin molding based head fixation technique for behavioral tasks in rodents.
Abstract
Head fixation is a technique of immobilizing animal’s head by attaching a head-post on the skull for rigid clamping. Traditional head fixation requires surgical attachment of metallic frames on the skull. The attached frames are then clamped to a stationary platform resulting in immobilization of the head. However, metallic frames for head fixation have been technically difficult to design and implement in general laboratory environment. In this study, we provide a novel head fixation method. Using a custom-made head fixation bar, head mounter is constructed during implantation surgery. After the application of acrylic resin for affixing implants such as electrodes and cannula on the skull, additional resins applied on top of that to build a mold matching to the port of the fixation bar. The molded head mounter serves as a guide rails, investigators conveniently fixate the animal’s head by inserting the head mounter into the port of the fixation bar. This method could be easily applicable if implantation surgery using dental acrylics is necessary and might be useful for laboratories that cannot easily fabricate CNC machined metal head-posts.
Introduction
头部固定或头部限制是通过在头骨的刚性夹紧附连框架或板固定动物的头的技术。该技术已通过精确的感官刺激，行为检测有或无神经记录清醒动物1-3和在睡眠-觉醒周期4 juxtacellular标签。它也可用于在体内脑成像5,6。现在，头部固定技术在神经科学和行为学研究中常用的工具。
基本上，传统的头部固定要求的金属头桩头骨6-8之上手术附件。附件随后夹紧到一固定平台，从而在头部的固定化。神经生理学研究在清醒的动物，如神经和/或光学记录，脑电路的干预，脑成像是伴随着使用牙科丙烯酸植入手术。因此，它似乎实现了头部固定在这些实验室的时候是实验需要一个简单的任务。帧应该被设计成重量轻和小到不会打扰在他们的家笼动物的自然行为。它也提供了机械刚性时夹紧在固定的平台。它进一步具有生物相容性取决于实验目的。因此，在技术上难以设计和制造在一般实验室设备，例如头职位。
在这项研究中，一种新的头部固定方法进行说明，提供了通过方便夹紧机械刚度，而无需使用金属头的职位。通过使用该方法，头部固定可以一般实验室条件下，其中，通常进行在啮齿类动物脑研究植入手术下进行。金属棒头桩被替换为丙烯酸树脂水泥由一个特制的头固定杆和模制工艺模制部分IMPLAN期间完成塔季翁手术。该固定杆提供了与其他植入物，如神经录像探头，刺激电极高空间的兼容性。还可以简化步骤通过除去螺钉紧固夹紧动物的头在固定平台上的。我们使用这种方法，在以前的行为研究和我们的结论是，这种方法很容易实现和方便，在实际的实验9。
Protocol
Representative Results
Discussion
This protocol demonstrates a simple and convenient head fixation method using materials at hand. For physically successful head fixation, the first critical steps during surgery is to rinse and dry the skull surface clearly before applying the dental adhesive resin cement. Second, this method does not uses screws implanting under temporal muscles and only depends on the screws on the skull, their perpendicular implantation against the surface of skull rim is crucial10. This reduces the possibility of accidenta…
Disclosures
The authors have nothing to disclose.
Acknowledgements
This research was supported by Basic Science Research Program through the NRF funded by the Ministry of Education, Science and Technology (2010-0022362) and (2012R1A2A2A02046812).
Materials
| Dental acrylic resin | Lang Dental Manufacturing | Ortho-Jet | to make molded mounter |
| Paraffin film | Bemis | Parafilm | to prevent adhesion of acrylic resin to the bar |
| Dental adhesive | Sun Medical | Super-Bond C&B | to bond between acrylic resin and skull |
| Plexglass or other acrylic plate | to make fixation bar and box platform | ||
| Cyanoacrylate | to bond acrylic plates |
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