Частица Агглютинация Метод Полиовирус Идентификация
Summary
Недавно был разработан новый частиц агглютинации (РА) анализ использования молекулы рецептора вируса позволило быстро и легко идентификации полиовируса (PV). В этой статье мы покажем, процедура анализа ПА для идентификации PV.
Abstract
In the Global Polio Eradication Initiative, laboratory diagnosis plays a critical role by isolating and identifying PV from the stool samples of acute flaccid paralysis (AFP) cases. In the World Health Organization (WHO) Global Polio Laboratory Network, PV isolation and identification are currently being performed by using cell culture system and real-time RT-PCR, respectively. In the post-eradication era of PV, simple and rapid identification procedures would be helpful for rapid confirmation of polio cases at the national laboratories. In the present study, we will show the procedure of novel PA assay developed for PV identification. This PA assay utilizes interaction of PV receptor (PVR) molecule and virion that is specific and uniform affinity to all the serotypes of PV. The procedure is simple (one step procedure in reaction plates) and rapid (results can be obtained within 2 h of reaction), and the result is visually observed (observation of agglutination of gelatin particles).
Protocol
Discussion
Единственной точкой этого анализа ПА использования PVR молекулы, а не анти-PV антитела для выявления PV 2. Это позволяет специфическим взаимодействием сенсибилизированных желатин частиц с PV с равномерной близости ко всем трем серотипов П. В. 3-6. Мы использовали форму immunoadhesin из PVR (PVR-IgG…
Disclosures
The authors have nothing to disclose.
Acknowledgements
Мы благодарны Junko Вада за отличную техническую помощь. Мы благодарны профессору Акио Номото за любезно предоставленные вектор бакуловирус выражение для PVR-IgG2a. Это исследование было частично поддержана грантами-в-помощь для содействия ликвидации полиомиелита и исследованиям в области новых и вновь возникающих инфекционных заболеваний из Министерства здравоохранения, труда и социального обеспечения.
Materials
| Name of the reagent | Company | Catalogue number | Comments |
|---|---|---|---|
| Sensitized-gelatin particles | Fujirebio Inc. | Test kit | |
| Reconstitution buffer | Fujirebio Inc. | Test kit | |
| Microtitration plate | Fujirebio Inc. | Test kit | |
| Anti-PV antibody | RIVM |
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