A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E
Published: April 30, 2024
Abstract
Source: Huang, Z., et al. Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE). J. Vis. Exp. (2019)
This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.
Protocol
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Basic information of the study group NOTE: The Allergy Information Repository of the State Key Laboratory of Respiratory Disease (AIR-SKLRD) is a large serum bank established inside the Guangzhou Institute of Respiratory Hospital (GIRH). Initiated in the last decade, the AIR-SKLRD has already started collecting and …
Disclosures
The authors have nothing to disclose.
Materials
| Agnitio BioIC Analyzer | Agnitio Science & Technology(Taiwan, China) | BA-G2000 | |
| BioIC Allergen specific-IgE Detection Kit | Agnitio Science & Technology(Taiwan, China) | DR17A12 | |
| BioIC Cartrideg Placement plate | Agnitio Science & Technology(Taiwan, China) | T20SET | |
| BioIC Reagent Dispenser | Agnitio Science & Technology(Taiwan, China) | DS-1 | |
| Image two-dimensional barcode machine | Agnitio Science & Technology(Taiwan, China) | NLS-HR200 | |
| VORTEX-5 Vortex Mixer | Haimen Kylinbell Lab Lastruments Co., Ltd. | VORTEX-5 |
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Cite This Article
A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E. J. Vis. Exp. (Pending Publication), e22119, doi: (2024).