A Molecular-Based Assay for Quantifying Influenza A and B Viruses
Published: February 29, 2024
Abstract
Source: Otto, C. C. et al., Rapid Molecular Detection and Differentiation of Influenza Viruses A and B. J. Vis. Exp. (2017)
The video demonstrates an assay for quantifying influenza viruses A and B. This assay employs nicking endonuclease amplification with influenza-specific primers and target detection using molecular beacon probes.
Protocol
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Before Running the Assay NOTE: The Influenza A and B assay is approved for nasopharyngeal swab specimens and for nasopharyngeal swabs stored in viral transport media. Swabs are included in the kit and should be used for optimal performance. However, rayon, foam, flocked swabs, or polyester nasal swabs can also be us…
Disclosures
The authors have nothing to disclose.
Materials
| Alere i Instrument | Alere | NAT-000 (Global), NAT-024 (US) | |
| Alere i Influenza A & B 24 Test Kit | Alere | 425-000 (Global), 425-024 (US) | |
| Alere i Barcode Scanner | Alere | EQ001001 | |
| Alere Universal Printer | Alere | 55115 (Global), alereiprinter (US) | |
| 200 µL precision pipette | |||
| 200 µL disposable pipette tips | |||
| Viral transport medium | Remel | M4-RT |
Tags
Cite This Article
A Molecular-Based Assay for Quantifying Influenza A and B Viruses. J. Vis. Exp. (Pending Publication), e22003, doi: (2024).