Generation of Chikungunya Virus-Like Particles Using Baculovirus Expression System in Insect Cells
Published: September 29, 2023
Abstract
Source: Arevalo, M. T. et al., Expression and Purification of Virus-like Particles for Vaccination. J. Vis. Exp. (2016)
This video shows how virus-like particles, VLPs are generated by infecting insect cells with a recombinant baculovirus carrying the chikungunya virus gene cassette. The recombinant virus infects cells, leading to VLP formation, assembly, and release into the culture medium for potential use in vaccine production.
Protocol
1. Chikungunya virus-like particles (VLP) Expression Using Baculovirus/Insect Cell System Generate recombinant baculovirus expressing chikungunya virus capsid and envelope proteins (C-E3-E2-6K-E1) from S-27 strain using a commercial baculovirus expression system. Culture Spodoptera frugiperda Sf9 cells in serum-free Sf9 growth medium with 100 U/ml penicillin and 100 µg/ml streptomycin at 28 °C. Use 3-5 x 105 c/ml to initiate spinner flask…
Disclosures
The authors have nothing to disclose.
Materials
| Opti-MEM I | Life Technologies | 31985062 | |
| Bac-to-Bac Baculovirus Expression System | Life Technologies | 10359-016 | |
| 0.22 μm vacuum filter top (500 mL) | Nalgene | 569-0020 | |
| Sf-900 II SFM | ThermoFisher Scientific | 10902-096 | |
| Cimarec I 6 multipoint stirrer plate | ThermoFisher Scientific | 50094596 |
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Cite This Article
Generation of Chikungunya Virus-Like Particles Using Baculovirus Expression System in Insect Cells. J. Vis. Exp. (Pending Publication), e21701, doi: (2023).