Microscale Thermophoresis to Study Protein-Lipid Interactions in Solution

Source: Sparks, R. P. et al., Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions. J. Vis. Exp. (2022)

This video demonstrates microscale thermophoresis for studying the protein-lipid interaction. The assay detects the interaction between molecules by quantifying the thermophoretic movement of fluorescent-labeled proteins in response to a temperature gradient. The fluorescent molecule is mixed with different concentrations of the non-fluorescent lipid molecules, and the mixture of molecules in the solution is loaded into capillaries. A temperature gradient is applied to the samples in the capillaries, and the movement of the fluorescent molecule in the temperature gradient is detected and recorded.