Rabbit Embryo Thawing: A Warming Procedure to Recover Cryopreserved Rabbit Embryos for Embryo Transfer
Published: April 30, 2023
Abstract
Source: Garcia-Dominguez, X. et al., Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model. J. Vis. Exp. (2019)
This video demonstrates the procedure for thawing vitrified rabbit embryos and the precautionary measurement steps for optimal embryo recovery.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Embryo Warming Thaw the embryos in a single step. Place the ministraw horizontally 10 cm from liquid nitrogen vapour for 20-30 s. When the crystallization process begins inside the ministraw, immerse the ministraw in a water bath at 25°C for 10-15 s. Remove the ministraw plug and cut the cotton plu…
Disclosures
The authors have nothing to disclose.
Materials
| Liquid Nitrogen | Air Liquide | P1505XXX | |
| Petri dishes, 35-mm | Sigma Aldrich | CLS430165-500EA | |
| Plastic dressing (Nobecutan) | IBOR medica | 7140028 | |
| Plastic Straw 0.25 mL | IMV – technologies | 6431 | |
| Scissors | ROBOZ | RS-5880 | Any regular surgical grade steel small straight scissors will work. |
| Sterile Gloves | Alvet Escartí | 087GL010075 | |
| Sterile gown | Alvet Escartí | 12261501 | |
| Sterile mask | Alvet Escartí | 058B15924B | |
| Straw Plug | IMV – technologies | 6431 | |
| Sucrose | Sigma Aldrich | S7903 | |
| Waterbath | RAYPA | BAE-4 | |
| Dulbecco's phosphate-buffered saline (DPBS) | Sigma Aldrich | D5773 | Without calcium chloride. |
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Cite This Article
Rabbit Embryo Thawing: A Warming Procedure to Recover Cryopreserved Rabbit Embryos for Embryo Transfer. J. Vis. Exp. (Pending Publication), e20936, doi: (2023).