Carotid Artery and Jugular Vein Interposition: A Technique to Induce Venous Intimal Hyperplasia in Rabbit Model
Published: April 30, 2023
Abstract
Source: Nishio, H., et al. A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure J. Vis. Exp. (2020).
This video demonstrates the interposition of the rabbit carotid artery with the jugular vein to induce venous intimal hyperplasia by subjecting the veins to arterial pressure. This model will help investigate molecular and genetic interventions to attenuate the progression of intimal hyperplasia after revascularization surgeries.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Interposing the carotid artery by the harvested jugular vein Begin with an anesthetized rabbit. Incise 50–60 mm of the cervical region with surgical scalpel longitudinally. Bluntly dissect the subcutaneous tissues and fascia. Expose a 20–30 mm segment of the ipsilateral carotid artery. Separate the artery carefully from…
Disclosures
The authors have nothing to disclose.
Materials
| 10% Povidone-iodine solution | Nakakita | 872612 | Surgical expendables |
| 2-0 VICRYL Plus | Johnson and Johnson | VCP316H | Surgical expendables |
| 4-0 Silk suture | Alfresa pharma | GA04SB | Surgical expendables |
| 8-0 polypropylene suture | Ethicon | 8741H | Surgical expendables |
| Fogarty Catheter (2Fr) | Edwards Lifesciences LLC | E-060-2F | Surgical expendables |
| Heparin | Nipro | 873334 | Anticoagulant |
| Intravenous catheter (20G) | Terumo | SR-OT2051C | Surgical expendables |
Tags
Cite This Article
Carotid Artery and Jugular Vein Interposition: A Technique to Induce Venous Intimal Hyperplasia in Rabbit Model. J. Vis. Exp. (Pending Publication), e20918, doi: (2023).