Murine Tail Lymphedema Model: An In Vivo Technique to Generate Sustained Lymphedema in Murine Tail
Published: April 30, 2023
Abstract
Source: Hassanein, A. H. et al., A Murine Tail Lymphedema Model. J. Vis. Exp. (2021).
This video describes an in vivo technique to generate murine tail lymphedema, an abnormal fluid accumulation in the interstitial tissue space leading to swelling in the tail. The model is applied to study mechanisms and therapy for lymphedema.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Surgical Disruption of Mouse Tail Lymphatics Use eight week-old C57BL/6 mice of equal gender distribution. Place a mouse under general anesthesia in an induction chamber with 3-4% isoflurane in 100% oxygen followed by maintenance sedation at 1-3% during the procedure. Administer 0.5 mg/kg sustained-r…
Disclosures
The authors have nothing to disclose.
Materials
| Surgical Microscope | Leica, Wetzlar, Germany | MSV266 | |
| Adherent Dressing (Tegaderm) | 3M, St. Paul, Minn. | 1626W | |
| C57BL/6 mice | Jackson Laboratories | 664 | |
| Micro-Adson Forceps – 1×2 Teeth | Fine Science Tools (USA) Inc. | 11019-12 | |
| Disposable Needle 30GX1 | Fischer Scientific | 305128 | |
| Operating Scissors | Fischer Scientific | 12-460-796 | |
| IsosulfanBlue (Lymphazurin) 50mg/5ml | Mylan | 67457-220-05 |
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Cite This Article
Murine Tail Lymphedema Model: An In Vivo Technique to Generate Sustained Lymphedema in Murine Tail. J. Vis. Exp. (Pending Publication), e20804, doi: (2023).