Implantation of Cranial Imaging Window in Mouse Model: A Surgical Procedure to Implant Glass-based Coverslip for Stable Optical Access to Regions of Murine Brain
Published: April 30, 2023
Abstract
Source: Alieva, M. et al. Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy. J. Vis. Exp. (2019)
This video presents a surgical procedure to implant cranial window in a mouse model for stable optical access to different regions of the brain.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Tumor Cell Implantation and Cranial Imaging Window Preparation Surgical Preparation Use adult mice (>6 weeks old) of any strain or gender. Sedate a mouse by injecting fluanisone [neuroleptic] + fentanyl [opioid]) (0.4 mL/kg) + benzodiazepine sedative (2 mg/kg) at a dose of 1:1:2 in steri…
Disclosures
The authors have nothing to disclose.
Materials
| 25G x 16 mm hypodermic needles | BD Microlance | ||
| Absorbable gelatin sponge | Pfizer | 300600 | |
| Coverslips round 6 mm | VWR international | Gelfoam | |
| Cyanoacrylate glue | Pattex | 631-0168 | Pattex Ultra gel |
| Dental cement | Vertex Dental | Vertex Self-Curing | |
| Drill | Dremel | Dremel 3000 (dental drill may be more convenient) + 105 Engraving Cutter | |
| Fine curved Tweezers | Dumont | ||
| Silicone Oil | Sigma Aldrich | AGT508 | |
| Stereotaxic frame | Stoelting | 181838 | |
| Surgical stereo microscope | Olympus | Lab standard stereotaxic, rat and mouse | |
| Opthalmic ointment | Kela Veterinaria | Duodrops veter kela 10 m | |
| Xylocaine (Lidocaine 1% + Epinephrine 1:100,000) Local anesthetic | Astrazeneca | Xylocaine (Lidocaine 1% + Epinephrine 1:100,000) | |
| Hypnorm | VetaPharma Ltd | Hypnorm (Fentanyl citrate 0.315 mg/mL + Fluanison 10 mg/mL) | |
| Midazolam | Actavis | Midazolam Actavis 5 mg/mL |
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Cite This Article
Implantation of Cranial Imaging Window in Mouse Model: A Surgical Procedure to Implant Glass-based Coverslip for Stable Optical Access to Regions of Murine Brain. J. Vis. Exp. (Pending Publication), e20625, doi: (2023).