Source: Chuan, Y. et al. Novel method of plasmid DNA delivery to mouse bladder urothelium by electroporation. J. Vis. Exp. (2018)
This video demonstrates the delivery of DNA plasmid into the urothelium of the mouse bladder using a combination of urethral catheterization and electroporation techniques. The described procedure allows the generation of mouse bladder cancer models.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Plasmid and Tools Preparation
2. Plasmid Delivery
3. Recovery
The authors have nothing to disclose.
BD 1mL TB Syringe | Fisher | 148232E | |
Buprenorphine | Sigma | B9275-50MG | |
Dumont Tweezers | Roboz Surgical Instr | RS-5005 | Treat with 70% ethanol before surgical use |
ECM830 SQ Wave Electroporator | Harvard Apparatus | 450052 | |
Exel International Disposable Safelet I.V. Catheters | Fisher Scientific | 14-841-21 | 24G, 2.11 cm length, 0.045 cm outer diameter |
Extra Fine Micro Dissecting Scissors | Roboz Surgical Instr | RS-5135 | Treat with 70% ethanol before surgical use |
Isoflurane | Vet one | 501017 | |
K&H Heated Resting Mat for Small Animals, 9 by 12 Inches | Amazon | Cover the heat mat with paper towels | |
Ophthalmic ointment | Fisher | NC0849514 | |
PBS, pH 7.4 | Life Technologies | 10010049 | |
Pipet tips 200 µL | USA Scientific | 1111-0206 | |
Pipet Tips, Universal Fit; 0.1 to 10 µL | Fisher | 02707438(CS) | |
PIPETMAN NEO P2 | Gilson | F144561 | |
PIPETMAN NEO P200N | Gilson | F144565 | |
plasmid CAG-GFP | Addgene | 16664 | |
Platinum tweezertrode 5 mm | Harvard Apparatus | 450489 | 5 mm diameter |
Scissors | Roboz Surgical Instr | RS-5880 | Treat with 70% ethanol before surgical use |
String cord | Amazon | Mandala Crafts 150D 210D 0.8mm 1mm leather sewing stitching flat waxed thread string cord | |
Tape, Scotch | Fisher | 19047257 | |
Therio-gel Veterinary Lubricant | PBS animal health | 353-736 | |
Trypan Blue Stain | Life Technologies | 15250061 | |
V-1 Table top system anesthesia | VetEquip | 901806 | |
Vicryl violet suture 18" FS-2 cutting 5-0 | Fisher | NC0578475 | |
Walgreens Povidone Iodine 10% | Walgreens | 953982 | |
Wound clip | Fisher | 18045 | |
Wound clip applier | Fisher | 1804 |