Bone Marrow Harvest from Mouse Hind Limb
Published: April 30, 2023
Abstract
Source: Zhu, Y. P., Padgett, et al. Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells. J. Vis. Exp. (2019).
This video describes a detailed protocol to isolate fresh whole bone marrow from the mouse's hind limbs to investigate whole BM defects for patients with blood disorders such as leukemia.
Protocol
1. Harvest Mouse Bone Marrow (BM) Purchase C57BL/6J mice from a commercial vendor. Feed the standard rodent chow diet and house in microisolator cages in a pathogen-free facility. Use male mice, 6-10 weeks of age, for experimental purpose. Euthanize by CO2 inhalation followed by the cervical dislocation. Place the mouse onto a sterile surgical pad with the abdomen side up. Sterilize the skin of the abdomen and hindlimbs area by spraying 70% ethanol. Use a pair o…
Disclosures
The authors have nothing to disclose.
Materials
| Mouse: C57BL/6J | The Jackson Laboratory | Stock No: 000664 | |
| HyClone Phosphate Buffered Saline solution | GE Lifesciences | Cat#SH30256.01 |
Cite This Article
Bone Marrow Harvest from Mouse Hind Limb. J. Vis. Exp. (Pending Publication), e20268, doi: (2023).