Systemic Pilocarpine Treatment: Mouse Model of Temporal Lobe Epilepsy
Published: April 30, 2023
Abstract
Source: Kim, J. E. and Cho, K. O. The Pilocarpine Model of Temporal Lobe Epilepsy and EEG Monitoring Using Radiotelemetry System in Mice. J. Vis. Exp. (2018).
This video presents the procedure for inducing status epilepticus (SE) in mice by systemic administration of pilocarpine to model temporal lobe epilepsy.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. SE Induction Purchase 8-week-old male C57BL/6NHsd mice and weigh each mouse. Then, use a marker pen to mark the tails of all mice for their easy identification during SE induction. Calculate the amount of scopolamine methyl bromide (scopolamine; 2 mg/kg), terbutaline hemisulfate (terbutaline; 2 mg/kg), and pil…
Disclosures
The authors have nothing to disclose.
Materials
| C57BL/6 | Envigo | C57BL/6NHsd | |
| Scopolamine methyl nitrate | Sigma | S2250 | Make 10X stock |
| Terbutaline hemisulfate salt | Sigma | T2528 | Make 10X stock |
| Pilocarpine hydrochloride | Sigma | P6503 | |
| Diazepam | SAMJIN | ||
| Castor oil (Kolliphor EL) | Sigma | C5135 | Polyoxyl 35 hydrogenated castor oil |
| Saline | Daihan pharm. Co. | ||
| 5% Dextrose | Daihan pharm. Co. | ||
| 1 mL syringe | Sung shim medial Co. Ltd. | ||
| 26 guage needle | Sung shim medial Co. Ltd. | 26 G * 13 mm (1/2") | |
| 30 guage needle | Sung shim medial Co. Ltd. | 30 G * 13 mm (1/2") |
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Cite This Article
Systemic Pilocarpine Treatment: Mouse Model of Temporal Lobe Epilepsy. J. Vis. Exp. (Pending Publication), e20030, doi: (2023).