JOVE JOURNAL

Medicine

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Journal (Medicine)

Diatom DNA Extraction from Water Sample and Human Lung Tissue Using a Modified DNA Extraction Kit

Yun-chao Zhou*¹, Bo Wang*¹, Jie Cai², Yu-zhao Xu¹, Xiao-shi Qin¹, Shan Ha¹, Bin Cong^1,3, Jian-hua Chen¹, Jian-qiang Deng¹

¹Hainan Province Tropical Forensic Engineering Research Center, Department of Forensic Medicine,Hainan Medical University, Hainan Provincial Academician Workstation (tropical forensic medicine), ²Hainan Vocational College of Political Science and Law, ³Department of Forensic Medicine,Hebei Medical University

Journal (Medicine)

Surgical Procedure for Intrapulmonary Tracheal Transplantation in Mice

Yamato Suzuki^1,2, Stephen Juvet^1,2, Mingyao Liu^1,2, Shaf Keshavjee^1,2

¹Latner Thoracic Research Laboratories, Toronto General Hospital Research Institute,University Health Network, ²Temerty Faculty of Medicine,University of Toronto

Journal (Medicine)

Murine Intrapulmonary Tracheal Transplantation: A Model for Investigating Obliterative Airway Disease After Lung Transplantation

Yamato Suzuki^1,2, Stephen Juvet^1,2, Mingyao Liu^1,2, Shaf Keshavjee^1,2

¹Latner Thoracic Research Laboratories, Toronto General Hospital Research Institute,University Health Network, ²Temerty Faculty of Medicine,University of Toronto

Journal (Medicine)

Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research

Hui-Chun Ku¹, Cheng-Wei Huang¹, Shih Yi Lee^2,3

¹Department of Life Science,Fu Jen Catholic University, ²MacKay Junior College of Medicine, Nursing, and Management, ³Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine,MacKay Memorial Hospital

Journal (Medicine)

Subclavian Vein Puncture Blood Collection and Observation of Secondary Outcome Measures in Rats

Xiao-hua Zhang*^1,2,3, Shun Peng*⁴, Zi-xu Pei⁵, Jing Sun⁶, Zhi-ping Wang^1,2,3

¹Institute of Urology,Lanzhou University Second Hospital, ²Key Laboratory of Gansu Province for Urological Diseases, ³Gansu Nephro-Urological Clinical Center, ⁴The First Clinical Medical College of Lanzhou University, ⁵The Second Clinical Medical College of Lanzhou University, ⁶Medical Experimental Center of Lanzhou University

Journal (Medicine)

Subclavian Vein Blood Sampling in Conscious Rats

Xiao-hua Zhang*^1,2,3, Shun Peng*⁴, Zi-xu Pei⁵, Jing Sun⁶, Zhi-ping Wang^1,2,3

¹Institute of Urology,Lanzhou University Second Hospital, ²Key Laboratory of Gansu Province for Urological Diseases, ³Gansu Nephro-Urological Clinical Center, ⁴The First Clinical Medical College of Lanzhou University, ⁵The Second Clinical Medical College of Lanzhou University, ⁶Medical Experimental Center of Lanzhou University

Journal (Medicine)

Model of Ischemia and Reperfusion Injury in Rabbits

Victor S. Alemany*¹, Dominic P. Recco*¹, Sitaram M. Emani¹, Pedro J. del Nido¹, James D. McCully¹

¹Department of Cardiac Surgery, Boston Children’s Hospital,Harvard Medical School

Journal (Medicine)

Cryopreservation of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells at the Optimal Stage

Xinyue Bai*^{1,2,3,4,5,6,7}, Ting Zhang*^{1,2,3,4,5,6,7}, Xinyue Zhu^{1,2,3,4,5,6,7}, Xianyu Huang^{1,2,3,4,5,6,7}, Haiyun Liu^{1,2,3,4,5,6,7}, Xiaoyan Ding⁸, Mei Jiang^{1,2,3,4,5,6,7}, Xiaodong Sun^{1,2,3,4,5,6,7}

¹Department of Ophthalmology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, ²National Clinical Research Center for Eye Diseases, ³Shanghai Clinical Research Center for Eye Diseases, ⁴Shanghai Key Clinical Specialty, ⁵Shanghai Key Laboratory of Ocular Fundus Diseases, ⁶Shanghai Engineering Center for Visual Science and Photomedicine, ⁷Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, ⁸Stem Cell Core Facility, CAS Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences

Journal (Medicine)

Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cell Dissociation and Optimal Cryopreservation Stage Determination

Xinyue Bai*^{1,2,3,4,5,6,7}, Ting Zhang*^{1,2,3,4,5,6,7}, Xinyue Zhu^{1,2,3,4,5,6,7}, Xianyu Huang^{1,2,3,4,5,6,7}, Haiyun Liu^{1,2,3,4,5,6,7}, Xiaoyan Ding⁸, Mei Jiang^{1,2,3,4,5,6,7}, Xiaodong Sun^{1,2,3,4,5,6,7}

¹Department of Ophthalmology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, ²National Clinical Research Center for Eye Diseases, ³Shanghai Clinical Research Center for Eye Diseases, ⁴Shanghai Key Clinical Specialty, ⁵Shanghai Key Laboratory of Ocular Fundus Diseases, ⁶Shanghai Engineering Center for Visual Science and Photomedicine, ⁷Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, ⁸Stem Cell Core Facility, CAS Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences

Journal (Medicine)

Preservation of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells with Optimized Cryopreservation

Xinyue Bai*^{1,2,3,4,5,6,7}, Ting Zhang*^{1,2,3,4,5,6,7}, Xinyue Zhu^{1,2,3,4,5,6,7}, Xianyu Huang^{1,2,3,4,5,6,7}, Haiyun Liu^{1,2,3,4,5,6,7}, Xiaoyan Ding⁸, Mei Jiang^{1,2,3,4,5,6,7}, Xiaodong Sun^{1,2,3,4,5,6,7}

¹Department of Ophthalmology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine, ²National Clinical Research Center for Eye Diseases, ³Shanghai Clinical Research Center for Eye Diseases, ⁴Shanghai Key Clinical Specialty, ⁵Shanghai Key Laboratory of Ocular Fundus Diseases, ⁶Shanghai Engineering Center for Visual Science and Photomedicine, ⁷Shanghai Engineering Center for Precise Diagnosis and Treatment of Eye Diseases, ⁸Stem Cell Core Facility, CAS Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences

Journal (Medicine)

Description of a Swine Infant Model of Volume-Controlled Hemorrhagic Shock

Alicia Rodríguez Martínez^1,2,3, Sara de la Mata Navazo^1,2,3, Gema Manrique Martín^1,2,3, Sarah Nicole Fernández Lafever^1,2,3, Laura Butragueño-Laiseca^1,2,3, María Slöcker Barrio^1,2,3, Rafael González Cortés^1,2,3, Laura Herrera Castillo^1,2,3, Santiago Mencía Bartolomé^1,2,3, Jimena del Castillo Peral^1,2,3, María José Solana García^1,2,3, Débora Sanz Álvarez^1,2,3, Raquel Cieza Asenjo^1,2,3, Jorge López-González^1,2,3, María José Santiago Lozano^1,2,3, Daniel Moreno Leira^1,2,3, Jesús López-Herce Cid^1,2,3, Javier Urbano Villaescusa^1,2,3

¹Pediatric Intensive Care Department,Gregorio Marañón University General Hospital, ²Gregorio Marañón Health Research Institute, ³Primary Care Interventions to Prevent Maternal and Child Chronic Diseases of Perinatal and Development Origin Network (RICORS),Carlos III Health Institute

Journal (Medicine)

Evaluating Diaphragm Thickness and Thickening Fraction During Tidal Breathing in Healthy Participants

Catherine A. Bellissimo¹, Idunn S. Morris^2,3,4, Jenna Wong¹, Ewan C. Goligher^1,2,3,5

¹Toronto General Hospital Research Institute, ²Interdepartmental Division of Critical Care Medicine,University of Toronto, ³Department of Physiology, Faculty of Medicine,University of Toronto, ⁴Deparatment of Intensive Care Medicine,Nepean Hospital, ⁵Division of Respirology, Department of Medicine,University Health Network

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