JOVE JOURNAL

Immunology and Infection

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Journal (Immunology and Infection)

Advanced Biochip Perfusion and Air-Liquid Interface Establishment of Human Umbilical Vein Endothelial Cells for Tissue Culture Analysis

Hristina Koceva¹, Mona Amiratashani¹, Knut Rennert², Alexander S. Mosig^1,3,4

¹Institute of Biochemistry II,Jena University Hospital, ²Dynamic42 GmbH, ³Center for Sepsis Control and Care,Jena University Hospital, ⁴Jena School of Microbial Communication,Friedrich Schiller University Jena

Journal (Immunology and Infection)

Non-Enzymatic Method for Isolating Neonatal Mouse Bone Marrow Cells

Madhavi Annamanedi¹, Jordan K. Vance¹, Cory M. Robinson^1,2

¹Department of Microbiology, Immunology, & Cell Biology,West Virginia University School of Medicine, ²Vaccine Development Center,West Virginia University Health Sciences Center

Journal (Immunology and Infection)

Differentiation of Neonatal Bone Marrow-Derived Macrophages Using a Supernatant of L929 Cells

Madhavi Annamanedi¹, Jordan K. Vance¹, Cory M. Robinson^1,2

¹Department of Microbiology, Immunology, & Cell Biology,West Virginia University School of Medicine, ²Vaccine Development Center,West Virginia University Health Sciences Center

Journal (Immunology and Infection)

Neonatal Mouse Bone Marrow Isolation and Preparation of Bone Marrow-Derived Macrophages

Madhavi Annamanedi¹, Jordan K. Vance¹, Cory M. Robinson^1,2

¹Department of Microbiology, Immunology, & Cell Biology,West Virginia University School of Medicine, ²Vaccine Development Center,West Virginia University Health Sciences Center

Journal (Immunology and Infection)

Assessing Phagocytic Function of Neonatal Bone Marrow-Derived Macrophages Using a Fluorescent Bacterial Uptake Assay

Madhavi Annamanedi¹, Jordan K. Vance¹, Cory M. Robinson^1,2

¹Department of Microbiology, Immunology, & Cell Biology,West Virginia University School of Medicine, ²Vaccine Development Center,West Virginia University Health Sciences Center

Journal (Immunology and Infection)

Harvesting and Seeding of Human Umbilical Vein Endothelial Cells (HUVECs) for Human Intestine-on-Chip Model

Adrian Feile*^1,2,3, Valentin D. Wegner*¹, Martin Raasch⁴, Alexander S. Mosig^1,2,3,5

¹Institute of Biochemistry II,Jena University Hospital, ²Cluster of Excellence Balance of the Microverse,Friedrich Schiller University, ³Jena School of Microbial Communication,Friedrich Schiller University, ⁴Dynamic42 GmbH, ⁵Jena Center for Sepsis Control and Care,Jena University Hospital

Journal (Immunology and Infection)

Microfluidic Perfusion of HUVEC and C2BBe1 Cultured Biochips for Analyzing Immune Responses to Microbial Colonization

Adrian Feile*^1,2,3, Valentin D. Wegner*¹, Martin Raasch⁴, Alexander S. Mosig^1,2,3,5

¹Institute of Biochemistry II,Jena University Hospital, ²Cluster of Excellence Balance of the Microverse,Friedrich Schiller University, ³Jena School of Microbial Communication,Friedrich Schiller University, ⁴Dynamic42 GmbH, ⁵Jena Center for Sepsis Control and Care,Jena University Hospital

Journal (Immunology and Infection)

Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses

Adrian Feile*^1,2,3, Valentin D. Wegner*¹, Martin Raasch⁴, Alexander S. Mosig^1,2,3,5

¹Institute of Biochemistry II,Jena University Hospital, ²Cluster of Excellence Balance of the Microverse,Friedrich Schiller University, ³Jena School of Microbial Communication,Friedrich Schiller University, ⁴Dynamic42 GmbH, ⁵Jena Center for Sepsis Control and Care,Jena University Hospital

Journal (Immunology and Infection)

Real-Time In Vitro Migration Assay for Primary Murine CD8+ T Cells

Allison T. Ryan^1,2, Ankit Dahal^1,2, Sana Mir^1,2, Minsoo Kim^1,2, Kihong Lim^1,2

¹Department of Microbiology and Immunology,University of Rochester, ²David H. Smith Center for Vaccine Biology and Immunology,University of Rochester

Journal (Immunology and Infection)

CD8+ T-Cell Purification from Mouse Lymph Nodes and Spleen for Kinetic Studies

Allison T. Ryan^1,2, Ankit Dahal^1,2, Sana Mir^1,2, Minsoo Kim^1,2, Kihong Lim^1,2

¹Department of Microbiology and Immunology,University of Rochester, ²David H. Smith Center for Vaccine Biology and Immunology,University of Rochester

Journal (Immunology and Infection)

Time-Lapse Imaging to Study Activated Murine CD8+ T-Cell Migration Dynamics

Allison T. Ryan^1,2, Ankit Dahal^1,2, Sana Mir^1,2, Minsoo Kim^1,2, Kihong Lim^1,2

¹Department of Microbiology and Immunology,University of Rochester, ²David H. Smith Center for Vaccine Biology and Immunology,University of Rochester

Journal (Immunology and Infection)

Tracking Migration of Activated T-Cells Isolated from Mouse Secondary Lymphoid Tissues

Allison T. Ryan^1,2, Ankit Dahal^1,2, Sana Mir^1,2, Minsoo Kim^1,2, Kihong Lim^1,2

¹Department of Microbiology and Immunology,University of Rochester, ²David H. Smith Center for Vaccine Biology and Immunology,University of Rochester

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