JOVE JOURNAL

Biology

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Lipid Supplementation for Longevity and Gene Transcriptional Analysis in Caenorhabditis elegans

Marzia Savini*^1,2, Yi-Tang Lee^2,3,4, Meng C. Wang^2,4,5, Yue Zhou*²

¹Graduate Program in Developmental Biology,Baylor College of Medicine, ²Huffington Center on Aging,Baylor College of Medicine, ³Integrative Program of Molecular and Biochemical Sciences,Baylor College of Medicine, ⁴Department of Molecular and Human Genetics,Baylor College of Medicine, ⁵Howard Hughes Medical Institute,Baylor College of Medicine

Journal (Biology)

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Aleksandra A. Petelski*¹, Nikolai Slavov^1,2,3, Harrison Specht*¹

¹Department of Bioengineering,Northeastern University, ²Barnett Institute,Northeastern University, ³Department of Biology,Northeastern University

Journal (Biology)

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis

Joseph Qian¹, Nicholas Ferrari¹, Richard Garcia¹, Mary Beth L. Rollins², Paul F. South^1,2

¹Department of Biological Sciences,Louisiana State University, ²Department of Plant Pathology and Crop Physiology,Louisiana State University AgCenter

Journal (Biology)

Long-Term Culture of Individual Caenorhabditis elegans on Solid Media for Longitudinal Fluorescence Monitoring and Aversive Interventions

Luis Espejo*¹, Bradford Hull*¹, Leah M. Chang¹, Destiny DeNicola¹, Samuel Freitas¹, Vanessa Silbar¹, Anne Haskins¹, Emily A. Turner¹, George L. Sutphin¹

¹Department of Molecular & Cellular Biology,University of Arizona, Tucson

Journal (Biology)

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Anjali Mahilkar*^1,2, Prachitha Nagendra*¹, Supreet Saini¹

¹Department of Chemical Engineering,Indian Institute of Technology Bombay, ²Department of Ecology and Evolutionary Biology,University of Michigan, Ann Arbor

Journal (Biology)

Assessing the Putative Anticryptococcal Properties of Crude and Clarified Extracts from Mollusks

Davier Gutierrez-Gongora¹, Fouad Raouf-Alkadhimi¹, Ryan S. Prosser², Jennifer Geddes-McAlister¹

¹Molecular and Cellular Biology Department,University of Guelph, ²School of Environmental Sciences,University of Guelph

Journal (Biology)

Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper (Capsicum annuum L.)

Chen Cheng¹, Jianxiu Liu¹, Zhiwen Wang¹, Jiahui Liu¹, Yaqin Wang¹, Yawen Liao¹, Zhiqiang Gao¹, Zhanjun Lu¹, Bo Zhu¹, Fengxian Yao¹

¹Life Sciences College,Gannan Normal University

Journal (Biology)

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Bilin Liu^1,2, Anqi Li¹, Yuan Qin³, Lei Chen¹, Meng Gao¹, Guohua Gong^1,2

¹Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology,Tongji University, ²Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences,Wenzhou Medical University, ³Department of Pharmacy, Shanghai East Hospital,Tongji University

Journal (Biology)

Tick Artificial Membrane Feeding for Ixodes scapularis

Benedict Khoo¹, Benjamin Cull², Jonathan D. Oliver¹

¹Division of Environmental Health Sciences, School of Public Health,University of Minnesota, ²Department of Entomology, College of Food, Agricultural and Natural Resources,University of Minnesota

Journal (Biology)

Extraction, Labeling, and Purification of Lineage-Specific Cells from Human Antral Follicles

Limor Man¹, Nicole Lustgarten Guahmich¹, Eleni Kallinos¹, Laury Arazi¹, Zev Rosenwaks^1,2, Daylon James^1,2

¹Center for Reproductive Medicine and Infertility,Weill Cornell Medical College, ²Tri-Institutional Stem Cell Derivation Laboratory,Weill Cornell Medical College

Journal (Biology)

The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

Nikolaos L. Dedousis¹, Lihong Teng¹, Alison B. Kohan¹

¹Division of Endocrinology and Metabolism, Department of Medicine, School of Medicine,University of Pittsburgh

Journal (Biology)

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Ying Wang^1,2, Zhuo An^1,2, Zhifang Zhao^1,2, Can Li³, Jiangbo Fan^1,2

¹Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology,Shanghai Jiao Tong University, ²Joint Center for Single Cell Biology, School of Agriculture and Biology,Shanghai Jiao Tong University, ³Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education),Shanghai Jiao Tong University

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