JOVE JOURNAL

Biology

Showing 637 - 648 in 3,291 videos.

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Shaohui Zheng^1,2, Fang Liang³, Yu Zhang^1,2, Ji-Feng Fei⁴, Wei Qin^1,2, Yanmei Liu^1,2

¹Key Laboratory of Brain, Cognition and Education Science,Ministry of Education, ²Institute for Brain Research and Rehabilitation, Guangdong Key Laboratory of Mental Health and Cognitive Science,South China Normal University, ³Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Engineering Technology Research Center for Environmentally Friendly Aquaculture, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Sciences,South China Normal University, ⁴Department of Pathology, Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences

Journal (Biology)

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Peibo Wang*¹, Yongcan Zhou*^1,3, Bei Wang², Ahmed Elaswad⁴, Shifeng Wang^1,3, Weiliang Guo^1,3, Dongdong Zhang^1,3

¹State Key Laboratory of Marine Resource Utilization in South China Sea,Hainan University, ²Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery,Guangdong Ocean University, ³Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science,Hainan University, ⁴Department of Animal Wealth Development, Faculty of Veterinary Medicine,Suez Canal University

Journal (Biology)

Ex Vivo Expansion and Genetic Manipulation of Mouse Hematopoietic Stem Cells in Polyvinyl Alcohol-Based Cultures

Hwei Minn Khoo*¹, Grace A. Meaker*¹, Adam C. Wilkinson¹

¹MRC Weatherall Institute of Molecular Medicine,University of Oxford

Journal (Biology)

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Elizabeth E. L. Lee¹, Emily Watto¹, Jocelyn Malamy¹

¹Department of Molecular Genetics and Cell Biology,The University of Chicago

Journal (Biology)

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Moirangthem Kiran Singh¹, Linda J. Kenney¹

¹Biochemistry & Molecular Biology,University of Texas Medical Branch

Journal (Biology)

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Devesh Shukla^1,2, Prabodh Kumar Trivedi^2,3, Shivendra Sahi^1,4

¹Department of Biology,Western Kentucky University, ²Plant Biotechnology Division,CSIR Central Institute of Medicinal and Aromatic Plants, ³Academy of Scientific and Innovative Research (AcSIR), ⁴Department of Biology,St. Joseph's University

Journal (Biology)

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Eloïse Mussard^1,2, Corinne Lencina¹, Gaëlle Boudry³, Caroline S. Achard², Christian Klotz⁴, Sylvie Combes¹, Martin Beaumont¹

¹GenPhySE,Université de Toulouse, INRAE, ENVT, ²Lallemand Animal Nutrition, ³Institut NuMeCan, INRAE, INSERM,Université de Rennes, ⁴FG 16: Mycotic and Parasitic Agents and Mycobacteria,Robert Koch-Institute

Journal (Biology)

High-Resolution Fluoro-Respirometry of Equine Skeletal Muscle

Michael Scott Davis¹, Montana Renae Barrett¹

¹Department of Physiological Sciences,Oklahoma State University

Journal (Biology)

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Marie Bannier-Hélaouët^1,2, Maarten H. Geurts^1,2, Jeroen Korving¹, Harry Begthel¹, Hans Clevers^1,2

¹Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW),University Medical Center Utrecht, ²Oncode Institute,Hubrecht Institute

Journal (Biology)

Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

Roberto Plebani^1,2, Alessandra D'Alessandro², Paola Lanuti^2,3, Pasquale Simeone^2,3, Massimiliano Cinalli¹, Ilaria Righi⁴, Alessandro Palleschi^4,5, Matteo Mucci^1,2, Marco Marchisio^2,3, Francesco Cappabianca¹, Marina Camera^6,7, Felice Mucilli¹, Mario Romano^1,2

¹Department of Medical, Oral and Biotechnological Sciences,“G. d’Annunzio” University of Chieti-Pescara, ²Center on Advanced Studies and Technology (CAST),“G. d’Annunzio” University of Chieti-Pescara, ³Department of Medicine and Aging Sciences,University “G. d’Annunzio” of Chieti-Pescara, ⁴U.O. di Chirurgia Toracica e dei Trapianti di Polmone,Fondazione IRCCS Ca’ Granda - Ospedale Maggiore Policlinico di Milano, ⁵Dipartimento di Fisiopatologia Medico-Chirurgica e dei Trapianti,Università degli Studi di Milano, ⁶Department of Pharmaceutical Sciences,Università degli Studi di Milano, ⁷Unit of Cell and Molecular Biology in Cardiovascular Diseases,Centro Cardiologico Monzino IRCCS

Journal (Biology)

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Michael Finnegan¹, Jeanne Hamet¹, Erick Desmarais², Stéphanie Bedhomme¹

¹CEFE,University of Montpellier, CNRS, EPHE, IRD, Montpellier, France, ²ISEM,University of Montpellier, CNRS, EPHE, IRD, Montpellier, France

Journal (Biology)

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Ana-María Figueroa¹, Francisca Stolzenbach¹, Pablo Tapia¹, Víctor Cortés¹

¹Department of Nutrition, Diabetes and Metabolism,Pontificia Universidad Católica de Chile

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