JOVE JOURNAL

Bioengineering

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Journal (Bioengineering)

Transfection and Harvesting Adeno-Associated Virus Vectors Using HEK293T Cells for Gene Therapy

Ting Zhang¹, Vinitha Dhamotharan¹, Yu Xiao^1,2, Sydne Ballengee^1,3, Jill Pollon^1,4, George K. Gittes¹

¹Division of Pediatric Surgery, Department of Surgery, Children's Hospital of Pittsburgh,University of Pittsburgh School of Medicine, ²North Allegheny High School, ³Wright State University Boonshoft School of Medicine, ⁴College of Engineering,University of Michigan

Journal (Bioengineering)

A Streamlined and Standardized Procedure for Generating High-Titer, High-Quality Adeno-Associated Virus Vectors Utilizing a Cell Factory Platform

Ting Zhang¹, Vinitha Dhamotharan¹, Yu Xiao^1,2, Sydne Ballengee^1,3, Jill Pollon^1,4, George K. Gittes¹

¹Division of Pediatric Surgery, Department of Surgery, Children's Hospital of Pittsburgh,University of Pittsburgh School of Medicine, ²North Allegheny High School, ³Wright State University Boonshoft School of Medicine, ⁴College of Engineering,University of Michigan

Journal (Bioengineering)

Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self-Assembling Peptide Matrix

Rosario Perez-Pedroza^1,2, Manola Moretti^1,2,3, Charlotte A. E. Hauser^1,2,3

¹Laboratory for Nanomedicine, Biological & Environmental Science & Engineering,King Abdullah University of Science and Technology, ²Computational Bioscience Research Center,King Abdullah University of Science and Technology, ³Red Sea Research Center,King Abdullah University of Science and Technology

Journal (Bioengineering)

SW1222 Cell Line-Derived Colorectal Cancer Organoid Fabrication in Ultrashort Self-Assembling Peptide Matrix

Rosario Perez-Pedroza^1,2, Manola Moretti^1,2,3, Charlotte A. E. Hauser^1,2,3

¹Laboratory for Nanomedicine, Biological & Environmental Science & Engineering,King Abdullah University of Science and Technology, ²Computational Bioscience Research Center,King Abdullah University of Science and Technology, ³Red Sea Research Center,King Abdullah University of Science and Technology

Journal (Bioengineering)

Immunostaining of Colorectal Cancer-Derived Organoids in Peptide Hydrogel

Rosario Perez-Pedroza^1,2, Manola Moretti^1,2,3, Charlotte A. E. Hauser^1,2,3

¹Laboratory for Nanomedicine, Biological & Environmental Science & Engineering,King Abdullah University of Science and Technology, ²Computational Bioscience Research Center,King Abdullah University of Science and Technology, ³Red Sea Research Center,King Abdullah University of Science and Technology

Journal (Bioengineering)

Imaging Immunostained Colorectal Cancer-Derived Organoids in Peptide Hydrogel for Evaluating the Effects of Self-Assembling Peptides on Cell Adhesion

Rosario Perez-Pedroza^1,2, Manola Moretti^1,2,3, Charlotte A. E. Hauser^1,2,3

¹Laboratory for Nanomedicine, Biological & Environmental Science & Engineering,King Abdullah University of Science and Technology, ²Computational Bioscience Research Center,King Abdullah University of Science and Technology, ³Red Sea Research Center,King Abdullah University of Science and Technology

Journal (Bioengineering)

Quantifying Inferior Vena Cava Compliance and Distensibility in an In Vivo Ovine Model Using 3D Angiography

Delaney J. Villarreal*^1,2, Tatsuya Watanabe*¹, Kirsten Nelson¹, Adrienne Morrison¹, Eric D. Heuer¹, Anudari Ulziibayar¹, John M. Kelly^1,3,4, Christopher K. Breuer^1,5

¹Center for Regenerative Medicine,Nationwide Children's Hospital, ²Biomedical Sciences Graduate Program,The Ohio State University College of Medicine, ³The Heart Center,Nationwide Children's Hospital, ⁴Department of Pediatrics,The Ohio State University College of Medicine, ⁵Department of Surgery,The Ohio State University College of Medicine

Journal (Bioengineering)

Catheterization of an Anesthetized Sheep for 3D Angiogram Analysis

Delaney J. Villarreal*^1,2, Tatsuya Watanabe*¹, Kirsten Nelson¹, Adrienne Morrison¹, Eric D. Heuer¹, Anudari Ulziibayar¹, John M. Kelly^1,3,4, Christopher K. Breuer^1,5

¹Center for Regenerative Medicine,Nationwide Children's Hospital, ²Biomedical Sciences Graduate Program,The Ohio State University College of Medicine, ³The Heart Center,Nationwide Children's Hospital, ⁴Department of Pediatrics,The Ohio State University College of Medicine, ⁵Department of Surgery,The Ohio State University College of Medicine

Journal (Bioengineering)

Acquisition of Pre-and Post-Bolus 3D Angiograms and Intravascular Pressure from a Catheterized Sheep for Measurement of the Vessel Circumference

Delaney J. Villarreal*^1,2, Tatsuya Watanabe*¹, Kirsten Nelson¹, Adrienne Morrison¹, Eric D. Heuer¹, Anudari Ulziibayar¹, John M. Kelly^1,3,4, Christopher K. Breuer^1,5

¹Center for Regenerative Medicine,Nationwide Children's Hospital, ²Biomedical Sciences Graduate Program,The Ohio State University College of Medicine, ³The Heart Center,Nationwide Children's Hospital, ⁴Department of Pediatrics,The Ohio State University College of Medicine, ⁵Department of Surgery,The Ohio State University College of Medicine

Journal (Bioengineering)

Tracking Fibrinolysis of Chandler Loop-Formed Whole Blood Clots Under Shear Flow in An In-Vitro Thrombolysis Model

Alexei Christodoulides¹, Abigail R. Hall¹, Anushri Umesh^1,2, Nathan J. Alves^1,2

¹Department of Emergency Medicine,Indiana University School of Medicine, ²Weldon School of Biomedical Engineering,Purdue University

Journal (Bioengineering)

Integrating Fluorescently Tagged Fibrinogen into Human Blood Clot for Enhanced Clot Visualization

Alexei Christodoulides¹, Abigail R. Hall¹, Anushri Umesh^1,2, Nathan J. Alves^1,2

¹Department of Emergency Medicine,Indiana University School of Medicine, ²Weldon School of Biomedical Engineering,Purdue University

Journal (Bioengineering)

Optimizing Real-Time Fluorometric Flow Loop System for Human Blood Clot Analysis

Alexei Christodoulides¹, Abigail R. Hall¹, Anushri Umesh^1,2, Nathan J. Alves^1,2

¹Department of Emergency Medicine,Indiana University School of Medicine, ²Weldon School of Biomedical Engineering,Purdue University

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