JOVE JOURNAL

Biochemistry

Showing 349 - 360 in 778 videos.

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Jan Wollenhaupt¹, Tatjana Barthel^1,2, Gustavo M. A. Lima³, Alexander Metz⁴, Dirk Wallacher⁵, Elmir Jagudin³, Franziska U. Huschmann^1,4, Thomas Hauß¹, Christian G. Feiler¹, Martin Gerlach¹, Michael Hellmig¹, Ronald Förster¹, Michael Steffien¹, Andreas Heine⁴, Gerhard Klebe⁴, Uwe Mueller¹, Manfred S. Weiss¹

¹Macromolecular Crystallography,Helmholtz-Zentrum Berlin, ²Structural Biochemistry Group, Institute for Chemistry and Biochemistry,Freie Universität Berlin, ³BioMAX,MAX IV Laboratory, ⁴Drug Design Group, Institute of Pharmaceutical Chemistry,Philipps-Universität Marburg, ⁵Department Sample Environment,Helmholtz-Zentrum Berlin

Journal (Biochemistry)

Fixed Target Serial Data Collection at Diamond Light Source

Sam Horrell¹, Danny Axford¹, Nicholas E. Devenish¹, Ali Ebrahim¹, Michael A. Hough², Darren A. Sherrell^1,3, Selina L. S. Storm^1,4, Ivo Tews⁵, Jonathan A. R. Worrall², Robin L. Owen¹

¹Diamond Light Source, Harwell Science and Innovation Campus, ²School of Life Sciences,University of Essex, ³X-ray Science Division,Argonne National Laboratory, ⁴European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, ⁵Biological Sciences, Institute for Life Sciences,University of Southampton

Journal (Biochemistry)

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

Changhua Zhu*¹, Xiaoye Liu*², Wenlei Wang¹, Xiaoguang Chen³, Shangyu Gao¹, Meng Qian¹, Na Yang¹, Yifeng Xu¹, Mingjia Chen¹

¹College of Life Sciences,Nanjing Agricultural University, ²Department of Criminal Science and Technology,Nanjing Forest Police College, ³Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition,Leibniz University Hannover

Journal (Biochemistry)

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

Alexander C. Y. Foo¹, Peter M. Thompson¹, Geoffrey A. Mueller¹

¹Nuclear Magnetic Resonance Group,National Institute of Environmental Health Sciences

Journal (Biochemistry)

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Noémie Mercier¹, Karine Prévost², Eric Massé², Pascale Romby¹, Isabelle Caldelari¹, David Lalaouna¹

¹Université de Strasbourg, CNRS, ²Department of Biochemistry,Université de Sherbrooke

Journal (Biochemistry)

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Di Wu¹, Grzegorz Piszczek¹

¹Biophysics Core Facility, National Heart, Lung, and Blood Institute,National Institutes of Health

Journal (Biochemistry)

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Candice M. Etson^1,2, Petar Todorov¹, Nooshin Shatery Nejad², Nirmala Shrestha², David R. Walt^1,3

¹Department of Chemistry,Tufts University, ²Department of Physics,Wesleyan University, ³Wyss Institute at Harvard University

Journal (Biochemistry)

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

Ananya Tripathi¹, Charles Bond^1,2, James R. Sellers¹, Neil Billington¹, Yasuharu Takagi¹

¹Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute,National Institutes of Health, ²Department of Physiology, Perelman School of Medicine,University of Pennsylvania

Journal (Biochemistry)

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

John H. Beale¹, May E. Marsh¹

¹Swiss Light Source,Paul Scherrer Institut

Journal (Biochemistry)

Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

Sean Rogers¹, W. Mike Henne¹

¹Department of Cell Biology,University of Texas Southwestern Medical Center

Journal (Biochemistry)

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Manabu Niimi¹, Haizhao Yan¹, Yajie Chen¹, Yao Wang², Jianglin Fan^1,2

¹Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine,University of Yamanashi, ²School of Biotechnology and Health Sciences,Wuyi University

Journal (Biochemistry)

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Mark D Tully*¹, Nicolas Tarbouriech², Robert P Rambo³, Stephanie Hutin*⁴

¹European Synchrotron Radiation Facility Structural Biology Group,Structural Biology Group, ²Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, ³Diamond Light Source, ⁴Laboratoire de Physiologie Cellulaire and Végétale,Université Grenoble Alpes/CNRS/CEA/INRA/BIG

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