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Coating CryoEM Grids with Monolayer Graphene to Improve Cryo-Sample Preparation

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単層グラフェンのクライオ電子顕微鏡グリッドへの高分解能構造決定への応用

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Application of Monolayer Graphene to Cryo-Electron Microscopy Grids for High-resolution Structure Determination

Please note that all translations are automatically generated. Click here for the English version.

単層グラフェンのクライオ電子顕微鏡グリッドへの高分解能構造決定への応用

Coating CryoEM Grids with Monolayer Graphene to Improve Cryo-Sample Preparation

DOI:

10.3791/66023-v

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01:38 min

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November 10, 2023

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Andrew V. Grassetti*¹, Mira B. May*¹, Joseph H. Davis²

¹Department of Biology,Massachusetts Institute of Technology, ²Program in Computational and Systems Biology,Massachusetts Institute of Technology

Tags

Keywords: Cryo-electron Microscopy Graphene Monolayer Sample Preparation Particle Localization Air-water Interface Grid Surface Sample Concentration Background Noise Affinity Handles Cell Lysates Cryogenic Electron Microscopy Holey Carbon Foil Vitreous Ice Image Processing Near-atomic Resolution Sample Preparation Bottleneck Graphene-coated Grids

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