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Biologia do Desenvolvimento

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실시간 이미징 of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

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실시간 이미징 of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

494 Views

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00:46 min

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October 13, 2023

DOI:

10.3791/65962-v

DOI:

10.3791/65962-v

•

00:46 min

October 13, 2023

•

2 Views

Roy Cohen², Danielle M. Sosnicki, Melissa A. White, Jacquelyn L. Nelson, Chinatsu Mukai, Alexander J. Travis²

¹Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Tags

Real-time Imaging Acrosomal Calcium Dynamics Sperm Exocytosis Calcium Signaling Capacitation Fertilization Membrane Lipids Acrosome-targeted Sensor For Exocytosis (AcroSensE)Genetically Encoded Calcium Indicator (GCaMP)High Frame-rate Imaging Sperm Activation Membrane Fusion CRISPR Gene-editing Transgenic Mouse Model

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