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Pulizia ottica ed etichettatura per la microscopia a fluorescenza a foglio luminoso nell'imaging cerebrale umano su larga scala

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Optical Clearing and Labeling for Light-sheet Fluorescence Microscopy in Large-scale Human Brain Imaging

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Pulizia ottica ed etichettatura per la microscopia a fluorescenza a foglio luminoso nell'imaging cerebrale umano su larga scala

1,092 Views

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01:20 min

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January 26, 2024

DOI:

10.3791/65960-v

DOI:

10.3791/65960-v

•

01:20 min

January 26, 2024

•

34 Views

Danila Di Meo*¹, Josephine Ramazzotti*¹, Marina Scardigli*^1,2, Franco Cheli, Luca Pesce³, Niamh Brady, Giacomo Mazzamuto^4,5, Irene Costantini^4,6, Francesco S. Pavone^4,5

¹European Laboratory for Non-linear Spectroscopy (LENS),University of Florence, ²Division of Physiology, Department of Experimental and Clinical Medicine,University of Florence, ³Department of Physics,University of Pisa, ⁴National Research Council – National Institute of Optics (CNR-INO), ⁵Department of Physics and Astronomy,University of Florence, ⁶Department of Biology,University of Florence

Tags

Optical Clearing Light-sheet Fluorescence Microscopy Large-scale Human Brain Imaging 3D Cytoarchitecture Volumetric Reconstruction Rapid Clearing Homogeneous Multi-labeling Neuronal Subpopulations Micrometer Resolution High-throughput Protocol

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