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Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

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哺乳類細胞の in situ クライオトモグラフィーのためのサンプル調製

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

Sample Preparation for In Situ Cryotomography of Mammalian Cells

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哺乳類細胞の in situ クライオトモグラフィーのためのサンプル調製

Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

DOI:

10.3791/65697-v

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01:19 min

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December 15, 2023

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Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Tags

Sample Preparation In Situ Cryotomography Mammalian Cells Electron Microscopy Grid Preparation Cell Attachment Cellular Protrusions Specialized Equipment Micro Patterning 3D Printed Holders Cellular Biology Virology Correlative Imaging

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