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小鼠常母离 体 肝机灌注
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JoVE Journal Engenharia
Normothermic Ex Vivo Liver Machine Perfusion in Mouse
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小鼠常母离 体 肝机灌注

DOI:
10.3791/65363-v

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13:14 min

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September 25, 2023

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, , , ,
1Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery,Jena University Hospital, 2Institute of Pathology,Klinikum Chemnitz GmbH, 3Department of Pathology, Faculty of Veterinary Medicine,Menoufia University

Capítulos

  • 00:09Introduction
  • 00:40Preparation
  • 04:33Liver Explanation
  • 09:49Liver and Chamber Connection
  • 10:53Adjust the Flow Rate According to PV Pressure
  • 11:19Representative Results
  • 12:23Conclusion

Summary

Tadução automática

Tadução automática

为小鼠肝脏创建了常温 离体 肝灌注（NEVLP）系统。该系统需要显微外科手术经验，但允许可重复的灌注结果。利用小鼠肝脏的能力有助于研究分子途径以鉴定新型灌注添加剂，并能够执行专注于器官修复的实验。

Tags

NormothermicEx-vivoLiverMachine PerfusionMouseMolecular MechanismsGenetically Modified AnimalsTransgenic MiceThermostatic ControllerPeristaltic PumpOxygenatorBubble TrapPortal VeinSuprahepatic CavaBile DrainageSemi-sterile30G Needle26G CatheterHeparinized SalineOrgan ChamberRoller PumpHeat Exchanger

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