This content is Free Access.
1Department of Molecular Biosciences,The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action,Michigan State University, 3Department of Microbiology and Molecular Genetics,Michigan State University, 4Department of Integrative Biology,Michigan State University, 5Biological Sciences Program,Michigan State University
Capítulos
- 00:03Introduction
- 02:02Daily Transfers of LTEE Populations
- 06:04Competitive Fitness Assays
- 06:44Prepare Supplies
- 08:24Day -2: Revive Competitors
- 09:09Day -1: Precondition Competitors
- 09:47Day 0: Begin Competition
- 11:16Alternative:Continue Three-Day Competition
- 11:43Day 1 or 3: Finish Competition
- 12:41Calculate and Plot Fitness
- 12:52Representative Results Appearance and turbidity of LTEE Cultures
- 13:22Representative Results Co-culture Competition Results
- 14:39Conclusion
Detta protokoll beskriver hur man upprätthåller Escherichia coli Long-Term Evolution Experiment (LTEE) genom att utföra sina dagliga överföringar och periodiska frysningar och hur man utför tävlingsanalyser för att mäta konditionsförbättringar i utvecklade bakterier. Dessa procedurer kan fungera som en mall för forskare som startar sina egna mikrobiella evolutionsexperiment.
