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1Department of Molecular Biosciences,The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action,Michigan State University, 3Department of Microbiology and Molecular Genetics,Michigan State University, 4Department of Integrative Biology,Michigan State University, 5Biological Sciences Program,Michigan State University
Capítulos
- 00:03Introduction
- 02:02Daily Transfers of LTEE Populations
- 06:04Competitive Fitness Assays
- 06:44Prepare Supplies
- 08:24Day -2: Revive Competitors
- 09:09Day -1: Precondition Competitors
- 09:47Day 0: Begin Competition
- 11:16Alternative:Continue Three-Day Competition
- 11:43Day 1 or 3: Finish Competition
- 12:41Calculate and Plot Fitness
- 12:52Representative Results Appearance and turbidity of LTEE Cultures
- 13:22Representative Results Co-culture Competition Results
- 14:39Conclusion
Questo protocollo descrive come mantenere l'esperimento di evoluzione a lungo termine (LTEE) di Escherichia coli eseguendo i suoi trasferimenti giornalieri e congelamenti periodici e come condurre test di competizione per misurare i miglioramenti della forma fisica nei batteri evoluti. Queste procedure possono servire come modello per i ricercatori che iniziano i propri esperimenti di evoluzione microbica.
