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Imunologia e Infecção

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Staining and Imaging to Visualize Neutrophil Extracellular Traps

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人和小鼠组织中性粒细胞胞外陷阱的免疫荧光成像

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Immunofluorescence Imaging of Neutrophil Extracellular Traps in Human and Mouse Tissues

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人和小鼠组织中性粒细胞胞外陷阱的免疫荧光成像

Staining and Imaging to Visualize Neutrophil Extracellular Traps

DOI:

10.3791/65272-v

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01:53 min

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August 18, 2023

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Lavinia Schoenfeld, Birgit Appl, Laia Pagerols-Raluy, Annika Heuer, Konrad Reinshagen, Michael Boettcher²

¹Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf,University of Hamburg, ²Department of Pediatric Surgery, University Medical Center Mannheim,University of Heidelberg, ³Division of Spine Surgery, Department of Trauma and Orthopedic Surgery,University Medical Center Hamburg-Eppendorf (UKE)

Tags

Keywords: Immunofluorescence Imaging Neutrophil Extracellular Traps NETs Antibody Selection Epitope Masking Autofluorescence Standardized Protocol Antigen Retrieval Permeabilization Citrullinated Histone 3 Autofluorescence-reducing Agent Electron Microscopy Autoimmune Diseases Sepsis Inflammation

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