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Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

Author Spotlight: The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

2,997 Views

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02:35 min

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April 07, 2023

DOI:

10.3791/65219-v

DOI:

10.3791/65219-v

•

02:35 min

April 07, 2023

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37 Views

Yuta Takase, Kazuto Fujishima³, Toshio Takahashi

¹Suntory Foundation for Life Sciences,Bioorganic Research Institute, ²Institute for Integrated Cell-Material Sciences (KUIAS-iCeMS),Kyoto University, ³Faculty of Medicine,Osaka Medical and Pharmaceutical University

Tags

3D Organoids Intestinal Crypts Intestinal Stem Cells Homeostasis Epithelial Growth Differentiation EDTA Mechanical Dissociation Signaling Intestinal Epithelium Paneth Cells Enteroendocrine Cells Enterocytes Goblet Cells Tuft Cells Leucine-rich Repeats Containing G Protein-coupled Receptor 5

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