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Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

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Analyse af fluorescerende farvede lipiddråber med 3D-rekonstruktion til vurdering af hepatisk steatose

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment

Analyse af fluorescerende farvede lipiddråber med 3D-rekonstruktion til vurdering af hepatisk steatose

Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

DOI:

10.3791/65206-v

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01:25 min

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June 02, 2023

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Karina Garcia^2,3,4, André Alves^2,3, Teresa M. Ribeiro-Rodrigues^2,3, Flávio Reis^2,3, Sofia Viana^2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

Tags

Lipid Droplets Hepatic Steatosis Microvesicular Steatosis Macrovesicular Steatosis BODIPY 493/503 Fluorescent Staining 3D Reconstruction Histological Assessment Liver High-fat Diet

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