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Eine optimierte quantitative Pull-Down-Analyse von RNA-bindenden Proteinen unter Verwendung kurzer biotinylierter RNA
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
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Eine optimierte quantitative Pull-Down-Analyse von RNA-bindenden Proteinen unter Verwendung kurzer biotinylierter RNA

1,958 Views

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07:55 min

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February 17, 2023

DOI:

10.3791/64923-v

DOI:
10.3791/64923-v

•

07:55 min
February 17, 2023

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5 Views
, , , ,
1Center for Human Technology,Italian Institute of Technology (IIT), 2Department of Experimental Oncology,European Institute of Oncology (IEO), 3Department of Biology and Biotechnologies ‘Charles Darwin’,Sapienza University of Rome

Tags

RNA-binding ProteinsPull-down AnalysisBiotinylated RNAProtein ComplexesHEK 293T CellsLysis BufferMagnetic BeadsYeast TRNARNA Oligonucleotide

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Crua Asensio, N., Rizzieri, S., Cuomo, A., Tartaglia, G. G., Zacco, E. An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA. J. Vis. Exp. (192), e64923, doi:10.3791/64923 (2023).

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