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使用胚胎海马神经元冷冻原液进行简单且可重复的低密度原代培养
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons
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使用胚胎海马神经元冷冻原液进行简单且可重复的低密度原代培养

1,911 Views

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04:26 min

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January 27, 2023

DOI:

10.3791/64872-v

DOI:
10.3791/64872-v

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04:26 min
January 27, 2023

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4 Views
, , , , ,
1Department of Pharmacology, Graduate School of Medicine,Gunma University, 2Gunma University Initiative for Advanced Research,Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences,The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc.

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Low-density Primary CultureFrozen StockEmbryonic Hippocampal NeuronsCell CulturePoly-L-lysineMicroelectrode ArrayCell SeedingCell SuspensionCytosine Beta-D-arabinofuranosideGlial Cell Growth

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Koganezawa, N., Roppongi, R. T., Sekino, Y., Tsutsui, I., Higa, A., Shirao, T. Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons. J. Vis. Exp. (191), e64872, doi:10.3791/64872 (2023).

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